Evaluation of the In vivo Radiosensitizing Activity of Etanidazole Using Tumor-bearing Chick Embryo
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概要
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Chick embryos have been used as alternative experimental animals in various research fields, including virology, immunology, toxicology, oncology, and embryology. Until now, there have been no in vivo models using chick embryo to evaluate radiosensitizing activity. Here, the in vivo radiosensitizing activity of etanidazole, a well-known hypoxic cell radiosensitizer, was evaluated using tumor-bearing chick embryo. On the basis of tumor growth, drug administration and X-ray irradiation were performed on day 15 chick embryo, with the endpoint being day 18 chick embryo. In day 15 chick embryo, an X-ray irradiation dose of equal or less than 10 Gy did not cause significant tumor growth suppression. Intravenous administration of equal or less than 1.0 mg of etanidazole did not cause tumor growth suppression. Neither doses of equal or less than 8 Gy of irradiation nor 1.0 mg of etanidazole caused fatality of the chick embryo. On the basis of these results, we evaluated the radiosensitizing effect of a combination treatment with 8 Gy of irradiation and 1.0 mg of etanidazole. As noted above, 1.0 mg of etanidazole alone and 8 Gy of irradiation alone did not show tumor growth suppression. In contrast, a combination treatment with 8 Gy of irradiation and 1.0 mg of etanidazole showed 35% of significant tumor growth suppression. Thus, we succeeded in evaluating the in vivo radiosensitizing activity of etanidazole using tumor-bearing chick embryo. These results suggest that the use of tumor-bearing chick embryo may be part of a promising system for evaluating radiosensitizing activity.
著者
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UTO Yoshihiro
Departmet of Biological Science and Technology, Faculty of Engineering, University of Tokushima
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HORI Hitoshi
Departmet of Biological Science and Technology, Faculty of Engineering, University of Tokushima
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MASUNAGA Shin-Ichiro
Particle Radiation Oncology Research Centre, Research Reactor Institute, Kyoto University
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NAKAE Takashi
Department of Mechanical Engineering, Toyama National College of Technology
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NAKATA Hiroko
Department of Surgery, Institute of Clinical Medicine, University of Tsukuba
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Nakata Eiji
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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ABE Chiaki
Department of Life System, Institute of Technology and Science, Graduate School, The University of T
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SHINMOTO Yuuya
Department of Life System, Institute of Technology and Science, Graduate School, The University of T
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SANO Keiichiro
Department of Life System, Institute of Technology and Science, Graduate School, The University of T
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TERAOKA Mizue
Department of Life System, Institute of Technology and Science, Graduate School, The University of T
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ENDO Yoshio
Central Research Resource Branch, Cancer Research Institute, Kanazawa University
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MAEZAWA Hiroshi
Institute of Health Biosciences, Graduate School, The University of Tokushima
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