Cynomolgus Macaque CYP4 Isoforms Are Functional, Metabolizing Arachidonic Acid
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概要
- 論文の詳細を見る
Cytochrome P450 (CYP) is important for metabolism of not only xenobiotics such as drugs, but also endogenous compounds including arachidonic acids. CYP4A11, CYP4F3v2, CYP4F11, and CYP4F45 have been identified in cynomolgus macaque, an animal species widely used for investigation of drug metabolism due to its evolutionary closeness to human. However, their metabolic functions have not been investigated. In this study, proteins were heterologously expressed in Escherichia coli and characterized by metabolic assays using arachidonic acids as substrates that are metabolized by CYP4 isoforms in human. The results showed that all four CYPs metabolized arachidonic acids. Therefore, cynomolgus macaque CYP4A11, CYP4F3v2, CYP4F11, and CYP4F45 are functional enzymes.
著者
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Yamazaki Hiroshi
Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University
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MATSUNO Kiyomi
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.
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UTOH Masahiro
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.
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NAKAMURA Chika
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.
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UNO Yasuhiro
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.
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Yamazaki Hiroshi
Laboratory Of Drug Metabolism And Pharmacokinetics Showa Pharmaceutical University
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Utoh Masahiro
Pharmacokinetics And Bioanalysis Center Shin Nippon Biomedical Laboratories Ltd.
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UNO Yasuhiro
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories
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MATSUNO Kiyomi
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories
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UTOH Masahiro
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories
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NAKAMURA Chika
Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories
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NAKAMURA Chika
Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL)
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UTOH Masahiro
Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL)
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MATSUNO Kiyomi
Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL)
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