Identification and Characterization of CYP2C18 in Cynomolgus Macaque (Macaca fascicularis)

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概要

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• The macaque is widely used for investigation in drug metabolism due to its evolutionary closeness to the human. However, genetic background of drug-metabolizing enzymes has not been fully investigated; therefore, identification and characterization of drug-metabolizing enzyme genes are important for understanding drug metabolism in this species. In this study, we isolated and characterized novel cytochrome P450 2C18 (CYP2C18) cDNA in cynomolgus macaques. This cDNA was highly homologous (96%) to human CYP2C18 cDNA. Cynomolgus CYP2C18 was preferentially expressed in liver and kidney. Moreover, the metabolic assay using cynomolgus CYP2C18 protein heterologously expressed in Escherichia coli revealed its activity toward S-mephenytoin 4’-hydroxylation. These results suggest that cynomolgus CYP2C18 could function as a drug-metabolizing enzyme in liver.

• 社団法人　日本獣医学会の論文

著者

• Yamazaki Hiroshi

  Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University

• MATSUNO Kiyomi

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• UTOH Masahiro

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• NAKAMURA Chika

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• UNO Yasuhiro

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• Yamazaki Hiroshi

  Laboratory Of Drug Metabolism And Pharmacokinetics Showa Pharmaceutical University

• Utoh Masahiro

  Pharmacokinetics And Bioanalysis Center Shin Nippon Biomedical Laboratories Ltd.

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