Identification and Characterization of CYP2B6 cDNA in Cynomolgus Macaques (Macaca fascicularis)

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概要

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• Cytochrome P450 2B6 (CYP2B6), an important drug-metabolizing enzyme, is involved in the metabolism of prescribed drugs in humans. Despite its importance, cDNA for a CYP2B6 ortholog has not been identified and characterized in cynomolgus macaques, which are frequently used in preclinical studies. In this study, cDNA highly homologous to human CYP2B6 was cloned from the cynomolgus macaque liver. This cDNA contained an open reading frame of 491 amino acids and functional domains characteristic for CYP protein, such as substrate recognition sites and a heme-binding region. Cynomolgus CYP2B6 was expressed predominantly in the liver with some extra-hepatic expression among 10 tissues. Moreover, cynomolgus CYP2B6 revealed activities toward testosterone 16β-hydroxylation and bupropion hydroxylation. These results suggest that cynomolgus CYP2B6 has a functional role in the liver.

• 社団法人　日本獣医学会の論文

著者

• Yamazaki Hiroshi

  Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University

• MATSUNO Kiyomi

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• UTOH Masahiro

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• NAKAMURA Chika

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• UNO Yasuhiro

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd.

• Yamazaki Hiroshi

  Laboratory Of Drug Metabolism And Pharmacokinetics Showa Pharmaceutical University

• Utoh Masahiro

  Pharmacokinetics And Bioanalysis Center Shin Nippon Biomedical Laboratories Ltd.

• UNO Yasuhiro

  Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories

• NAKAMURA Chika

  Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL)

• UTOH Masahiro

  Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL)

• MATSUNO Kiyomi

  Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL)

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