Differential Diagnosis of Feline Leukemia Virus Subgroups Using Pseudotype Viruses Expressing Green Fluorescent Protein
スポンサーリンク
概要
- 論文の詳細を見る
Feline leukemia virus (FeLV) is classified into three receptor interference subgroups, A, B and C. In this study, to differentiate FeLV subgroups, we developed a simple assay system using pseudotype viruses expressing green fluorescent protein (GFP). We prepared gfp pseudotype viruses, named gfp(FeLV-A), gfp(FeLV-B) and gfp(FeLV-C) harboring envelopes of FeLV-A, B and C, respectively. The gfp pseudotype viruses completely interfered with the same subgroups of FeLV reference strains on FEA cells (a feline embryonic fibroblast cell line). We also confirmed that the pseudotype viruses could differentiate FeLV subgroups in field isolates. The assay will be useful for differential diagnosis of FeLV subgroups in veterinary diagnostic laboratories in the future.
- 社団法人 日本獣医学会の論文
著者
-
BABA Kenji
Laboratory of Veterinary Parasitology, Department of Veterinary Medicine, Faculty of Agriculture, Ya
-
MIYAZAWA Takayuki
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto U
-
Miura Tomoyuki
Laboratory of Primate Model, Experimental Research Center for Infectious Disease, Institute for Viru
-
Nakamura Megumi
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto U
-
Sato Eiji
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto U
-
SHIMODA Tetsuya
SANYO Animal Medical Center
-
Miura Tomoyuki
Laboratory Of Pathogenic Virus Institute For Virus Research Kyoto University
-
Nakamura Megumi
Laboratory Of Chemistry And Biochemistry Osaka Prefectural College Of Health Sciences
-
Baba Kenji
Laboratories Of Veterinary Parasitology Dep. Of Veterinary Medicine Fac. Of Agriculture Yamaguchi Univ. 1677-1 ...
-
BABA Kenji
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University
-
MIURA Tomoyuki
Laboratory of Primate Model, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University
関連論文
- Isolation of Koala Retroviruses from Koalas in Japan
- Prekallikrein Deficiency in a Dog
- Peripheral Blood CD4 and CD8 Double-Positive T Cells of Rhesus Macaques Become Vulnerable to Simian Immunodeficiency Virus by In Vitro Stimulation Due to the Induction of CCR5
- In vitro and in vivo stability of plasmids in attenuated Salmonella enterica serovar typhimurium used as a carrier of DNA vaccine is associated with its replication origin
- Construction and In Vitro Properties of Chimeric Simian and Human Immunodeficiency Virus with the Human TNF-Alpha Gene
- Successive Deaths of a Captive Snow Leopard (Uncia uncia) and a Serval (Leptailurus serval) by Infection with Feline Panleukopenia Virus at Sapporo Maruyama Zoo
- Differential Diagnosis of Feline Leukemia Virus Subgroups Using Pseudotype Viruses Expressing Green Fluorescent Protein
- Intrathymic Effect of Acute Pathogenic SHIV Infection on T-Lineage Cells in Newborn Macaques
- PHYLOGENETIC ANALYSIS OF HTLV-1 AND STLV : The Past Movement of HTLV-1-Carriers and the Origin of PTLV
- Prevalence and Phylogenetic Analysis of HTLV-I Isolates in Cameroon, Including Those of the Baka Pygmy
- Isolation of STLV-I from Orangutan, a Great Ape Species in Southeast Asia, and Its Relation to Other HTLV-Is/STLV-Is
- Effects of PMA and Transcription Factors on Ovine Interferon-τ Transactivation in Various Cell Lines
- Unusual Permeability of Porcine Endogenous Retrovirus Subgroup A through Membrane Filters
- Virology: Isolation of koala retroviruses from koalas in Japan
- Acute Myelomonocytic Leukemia (AML-M4) in a Dog with the Extradural Lesion
- Focus Assay on FeLIX-Dependent Feline Leukemia Virus
- Neutropenia Associated with Osteomyelitis due to Hepatozoon canis Infection in a Dog
- Focus Assay on RD114 Virus in QN10S Cells(Virology)
- BASIC Programs for Calculating the Composition of the Solution Containing Multiple Metals and Ligands Used for Experiments in Ion Transport Systems
- Identification and Characterization of Feline UBE1L Gene
- Quantitative Assays for Xenotropic Murine Leukemia Virus-Related Virus
- Presence of Infectious RD-114 Virus in a Proportion of Canine Parvovirus Isolates
- Development of High-Grade B-Cell Lymphoma Concurrent with T-Cell Chronic Lymphocytic Leukemia in a Dog
- Molecular Detection of Spotted Fever Group Rickettsia in Feral Raccoons (Procyon lotor) in the Western Part of Japan
- Ehrlichia canis Infection in Two Dogs that Emigrated from Endemic Areas
- Focus Assay on RD114 Virus in QN10S Cells
- A Hematological Study on Thirteen Cats with Myelodysplastic Syndrome.
- Chronic Myelomonocytic Leukemia in a Cat.