In vitro and in vivo stability of plasmids in attenuated Salmonella enterica serovar typhimurium used as a carrier of DNA vaccine is associated with its replication origin

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概要

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• The ability of live attenuated Salmonella enterica serovar typhimurium (S. typhimurium) as a carrier of DNA vaccine was evaluated using model plasmid encoding beta-galactosidase (β-Gal) and BALB/c mice. We constructed pBRCMVβ, β-Gal expression apparatus having a replication origin from low copy pBR322. Comparison of the plasmid stability showed that pBRCMVβ remained stable in Salmonella even after oral administration, while pUC-based pCMVβ tended to be lost quickly. However, titers for β-Gal specific IgG in sera did not significantly increase in mice orally administered S. typhimurium harboring pBRCMVβ. These data suggest that the stability of plasmid in S. typhimurium is associated with its replication origin. Further studies are required to scientifically establish this methodology.

• Japanese Association for Laboratory Animal Science,社団法人　日本実験動物学会の論文
• 2006-07-01

著者

• 清水 佑也

  宮崎大学農学部獣医微生物学講座

• Miura Tomoyuki

  Laboratory of Primate Model, Experimental Research Center for Infectious Disease, Institute for Viru

• GOTO Yoshitaka

  Department of Veterinary Microbiology, University of Miyazaki

• HAGA Takeshi

  Department of Veterinary Microbiology, University of Miyazaki

• KUMABE Shino

  Department of Veterinary Microbiology, University of Miyazaki

• IKEJIRI Ai

  Department of Veterinary Microbiology, University of Miyazaki

• SHIMIZU Yuya

  Department of Veterinary Microbiology, University of Miyazaki

• LI Huang

  Department of Veterinary Microbiology, University of Miyazaki

• MATSUI Hidenori

  Kitasato Institute for Life Sciences, Kitasato University

• MIYATA Hironori

  Animal Research Center, University of Occupational and Environmental Health

• Miura Tomoyuki

  Laboratory Of Viral Pathogenesis Institute For Virus Research Kyoto University

• Ikejiri Ai

  Department Of Veterinary Microbiology University Of Miyazaki

• Haga Takeshi

  Department Of Veterinary Microbiology University Of Miyazaki

• Miura Tomoyuki

  Laboratory Of Pathogenic Virus Institute For Virus Research Kyoto University

• Kumabe Shino

  Department Of Veterinary Microbiology University Of Miyazaki

• Haga Takeshi

  Department Of Veterinary Microbiology Miyazaki University

• Shimizu Yuya

  Department Of Veterinary Microbiology University Of Miyazaki

• Miura Tomoyuki

  Laboratory Of Primate Model Experimental Research Center For Infectious Disease Institute For Virus

• Goto Yoshitaka

  Department Of Veterinary Microbiology University Of Miyazaki

• Goto Yoshitaka

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Haga Takeshi

  Univ. Miyazaki Miyazaki

• Hayami Masanori

  Kitasato Institute For Life Sciences Kitasato University

• Matsui Hidenori

  Kitasato Institute For Life Sciences Kitasato University

• Li Huang

  Department Of Veterinary Microbiology University Of Miyazaki

• 芳賀 猛

  Department of Veterinary Microbiology, University of Miyazaki

• 隈部 志野

  Department of Veterinary Microbiology, University of Miyazaki

• 池尻 藍

  Department of Veterinary Microbiology, University of Miyazaki

• Matsui Hidenori

  Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University

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