Initial Detection of a Circular Genome Deletion in a Naturally Bovine Papillomavirus-Infected Sample
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概要
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Bovine papillomavirus type 12 (BPV-12) was recently identified in epithelial papillomas on the cattle tongue in our previous study. Along with the full-length genome, one deleted circular genome, named BPV-12-del, was detected in the same papilloma lesion. BPV-12-del is 3363 base pairs in length, and a total of 3384 base pairs, including E1, E2 and E4 genes and partial E7 and L2 ORFs, were deleted compared with the complete genome. Real-time PCR results showed that the percentage of BPV-12-del was 42% of the total genomes in the sample. Southern Blot analysis also confirmed the presence of the deleted genome. This is the first report describing a circular genome deletion detected in a naturally BPV-infected sample.
著者
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DONG Jianbao
Department of Veterinary Microbiology, University of Miyazaki
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Goto Yoshitaka
Department Of Applied Chemistry Faculty Of Engineering Oita University
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Zhu Wei
Department Of Pathology Cancer Hospital Shanghai Medical University
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GOTO Yoshitaka
Department of Veterinary Microbiology, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan
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HAGA Takeshi
Division of Infection Control and Disease Prevention, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-Ku, Tokyo 113–8657, Japan
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ZHU Wei
Department of Veterinary Microbiology, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan
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