Rapid and Sensitive Detection of Bovine Coronavirus and Group A Bovine Rotavirus from Fecal Samples by Using One-Step Duplex RT-PCR Assay
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概要
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Bovine coronavirus (BCoV) and group A bovine rotavirus (BRV) are two of major causes for neonatal calf diarrhea. In the present study, a one-step duplex RT-PCR was established to detect and differentiate BCoV and group A BRV from fecal samples. The sensitivity of this method for BCoV and group A BRV was 10 PFU/100 μl and 1 PFU/100 μl, respectively. Twenty-eight diarrhea fecal samples were detected with this method, the result showed that 2 samples were identified as co-infected with BCoV and group A BRV, 26 samples were group A BRV positive, and 2 samples were negative. It proved that this method is sensitive for clinical fecal samples and is worth applying to laboratory diagnosis for BCoV and group A BRV.
著者
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GOTO Yoshitaka
Department of Veterinary Microbiology, University of Miyazaki
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HAGA Takeshi
Department of Veterinary Microbiology, University of Miyazaki
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ZHU Wei
Department of Veterinary Microbiology, University of Miyazaki
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DONG Jianbao
Department of Veterinary Microbiology, University of Miyazaki
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SUEYOSHI Masuo
Department of Veterinary Hygiene, University of Miyazaki
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Haga Takeshi
Department Of Veterinary Microbiology Miyazaki University
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Goto Yoshitaka
Department Of Applied Chemistry Faculty Of Engineering Oita University
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Zhu Wei
Department Of Pathology Cancer Hospital Shanghai Medical University
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Haga Takeshi
Department of Microbiology, University of Miyazaki
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Sueyoshi Masuo
Department of Veterinary Hygiene, Faculty of Agriculture, Miyazaki University
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