Identification of Weakly Beta-Hemolytic Porcine Spirochetes by Biochemical Reactions, PCR-Based Restriction Fragment Length Polymorphism Analysis and Species-Specific PCR

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概要

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• We examined the usefulness of PCR-based restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (WBHIS) isolated from pigs. WBHIS strains showing atypical biochemical characteristics were decisively identified at the species level by PCR-RFLP and species-specific PCR. Identification of WBHIS at the species level in routine diagnostic work will certainly contribute to clarifying the pathogenicity of WBHIS.

• 公益社団法人 日本獣医学会の論文

著者

• OHYA Tatsuo

  Kyushu Research Station, Environmental/Enzootic Diseases Research Team, National Institute of Animal

• Sueyoshi Masuo

  Department of Veterinary Hygiene, Faculty of Agriculture, Miyazaki University

• OHYA Tatsuo

  Kyushu Research Station, Environmental/Enzootic Diseases Research Team, National Institute of Animal Health

• ARAKI Hiroshi

  Nagasaki Prefecture Kenhoku Livestock Hygiene Service Center

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• Effect of Bicozamycin on the Eradication of Shiga Toxin-Producing Escherichia coli in Calves
• Antimicrobial Susceptibilities of Shiga Toxin-Producing Escherichia coli Isolates from Pigs with Edema Disease in Japan
• Identification of Weakly Beta-Hemolytic Porcine Spirochetes by Biochemical Reactions, PCR-Based Restriction Fragment Length Polymorphism Analysis and Species-Specific PCR

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