Identification and Characterization of Feline UBE1L Gene
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概要
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Interferon-stimulated gene 15 (ISG15) is one of the type I interferon-inducible proteins. Addition of ISG15 known as ISGylation is an ubiquitin-like posttranslational modification. Coexpression of ISG15 and ubiquitin-activating enzyme E1-like protein (UBE1L) is required to induce ISGylation. Previously, we identified feline ISG15 gene and found that the capsid protein of feline immunodeficiency virus was ISGylated in vitro by treatment with feline interferon-ω. In this study, we cloned feline UBE1L (FeUBE1L) gene to further study the mechanism of the antiviral activities induced by ISGylation. Sequencing analysis revealed that active sites of FeUBE1L were highly conserved. These data suggest that FeUBE1L has an enzymatic activity. Further, expression of FeUBE1L was induced in feline cell lines by treatment with feline interferon-ω and ovine interferon-τ.
著者
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MIYAZAWA Takayuki
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto U
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KOBAYASHI TAKESHI
Laboratory of Applied Microbiology, Graduate School of Marine Science and Technology, Tokyo Universi
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MIYAZAWA Takayuki
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University
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SATO Hisaaki
Laboratory of Veterinary Microbiology, Faculty of Veterinary Medicine, School of Veterinary Medicine, Kitasato University
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TANABE Taishi
Laboratory of Veterinary Microbiology, Faculty of Veterinary Medicine, School of Veterinary Medicine, Kitasato University
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SHIMODE Sayumi
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University
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