Focus Assay on RD114 Virus in QN10S Cells(Virology)
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概要
- 論文の詳細を見る
Cats harbor an infectious endogenous retrovirus, named RD114 virus. It is therefore necessary to monitor RD114 virus production in feline cells which are used for biological products as substrates. In this study, a feline sarcoma-positive leukemia-negative (S+L-) fibroblast cell line, named QN10S cells, was found to be highly susceptible to RD114 pseudotype viruses. The cells were transformed by infection with RD114 virus and the numbers of foci could be counted. The sensitivity of the focus assay was lower than that of the LacZ marker rescue assay in detecting RD114 virus. Although the assay is not suitable to detect a small amount of the virus, the assay will be useful for virological studies of RD114 virus.
- 社団法人日本獣医学会の論文
- 2008-12-25
著者
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BABA Kenji
Laboratory of Veterinary Parasitology, Department of Veterinary Medicine, Faculty of Agriculture, Ya
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MIYAZAWA Takayuki
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto U
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SHOJIMA Takayuki
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto U
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YOSHIKAWA Rokusuke
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto U
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SAKAGUCHI Shoichi
Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research,
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ISHIKAWA Mieko
Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research,
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Baba Kenji
Laboratory Of Viral Pathogenesis Center For Emerging Virus Research Institute For Virus Research Kyo
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Ishikawa Mieko
Laboratory Of Viral Pathogenesis Center For Emerging Virus Research Institute For Virus Research Kyo
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Ishikawa Mieko
Laboratory Of Chemistry Faculty Of Education Kanazawa University
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Sakaguchi Shoichi
Kyoto Univ. Kyoto
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Baba Kenji
Laboratories Of Veterinary Parasitology Dep. Of Veterinary Medicine Fac. Of Agriculture Yamaguchi Univ. 1677-1 ...
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Sakaguchi Shoichi
Laboratory Of Viral Pathogenesis Center For Emerging Virus Research Institute For Virus Research Kyoto University
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SHOJIMA Takayuki
Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University
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