BASIC Programs for Calculating the Composition of the Solution Containing Multiple Metals and Ligands Used for Experiments in Ion Transport Systems
スポンサーリンク
概要
- 論文の詳細を見る
The present paper deals with the method used for computing the composition of the solution used for the experiments in isolated cellular organelles, such as isolated sarcoplasmic reticulum or isolated contractile proteins. In these systems, calcium contamination of the solution is usually sufficient to activate the enzymes, since they are controlled by low concentrations of Ca^<2+> ion. The free concentration of other ions, such as magnesium, are also important. These required free ion concentrations may be obtained by the use of metal buffers, composed of the metal ion and an appropriate ligand. Taking into account the formation of complexes between multiple metals and ligands, we developed BASIC language programs for the calculation of the concentrations of the components. Three programs are presented. The first and the second calculate the apparent binding constants of complexes between metals and ligands at a specified pH by means of absolute binding constants. The third one computes the total concentrations of metals and ligands necessary to obtain each concentration of specified free metal ions or other components in the presence of more than one metal and ligand. One of the major advantages of a BASIC language program is its availability to investigators without any experience in computer programing. We intend to explain the equations and rationale used, in sufficient detail, so that any investigator will be able to adopt the BASIC programs proposed here to a personal computer, of which the operating system is MS-Windows 95 or 98.
- 大阪府立大学の論文
- 1999-01-29
著者
-
Nakamura Hiroshi
Laboratory of Animal Behavior and Intelligence, Division of Biological Sciences, Graduate School of
-
Nakamura Megumi
Laboratory Of Chemistry And Biochemistry Osaka Prefectural College Of Health Sciences
関連論文
- PKA-Dependent Regulation of Synaptic Enhancement between a Buccal Motor Neuron and Its Regulatory Interneuron in Lymnaea stagnalis
- Differential Diagnosis of Feline Leukemia Virus Subgroups Using Pseudotype Viruses Expressing Green Fluorescent Protein
- Operant Conditioning of Escape Behavior in the Pond Snail, Lymnaea stagnalis(Physiology)
- Neuronal components of the superior and inferior tentacles in the terrestrial slug, Limax marginatus
- BASIC Programs for Calculating the Composition of the Solution Containing Multiple Metals and Ligands Used for Experiments in Ion Transport Systems
- Purification, Properties and Recognition Sequence of Site-specific Restriction Endonuclease from "Acetobacter xylinus"