Light scattering from assemblies of an archaeal feast/famine regulatory protein, DM1 (pot1216151)

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Light scattered by assemblies of a feast/famine regulatory protein (FFRP), pot1216151 (DM1) from the hyperthermophilic archaeon Pyrococcus sp. OT3, was measured by combining an multi-angle detector with a gel filtration device. Four peaks were separated by gel filtration. For peak 4, the molecular weight (MW) was estimated as ~20K, a value not so different from that of dimers, 17K, by measuring static light scattering. For peak 3, MW was estimated as ~33K, suggesting formation of tetramers of MW of 34K. Peak 2 was the smallest among the four peaks, and not clearly separated from nearby peak 3, upon measuring light scattering. While, MW estimated for peak 1 was 112-118K, an intermediate between that of octamer, 69K, and that of hexadecamers, 138K. On the basis of these findings transition between assembly forms is discussed. (Communicated by Masanori OTSUKA, M.J.A.)
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