An electron microscopic study of the archaeal feast/famine regulatory protein : 5. Fourier filtration of images
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概要
- 論文の詳細を見る
Microcrystals of the feast/famine regulatory protein (FFRP) pot0434017 (FL11) were prepared by sonicating larger crystals. Using the microcrystals a cryo-electron micrograph was obtained, which showed a hexagonal packing of cylinder-like assemblies of FL11. This micrograph was processed by selecting, in the Fourier space, spots reflecting the crystal lattice, thereby removing the noise. The microcrystal was not totally free from distortion, and cylinders in local clusters adopted slightly different orientations. Thus, 25 hexagonal units closest to the ideal, each containing a cylinder at the center surrounded by six others, were manually selected. The averaged image was further processed to yield a perfect six-fold symmetry. These processed images, and some of the original images too, show bridges connecting cylinders, each corresponding to two pairs of N-domains, protruding from the two cylinders and contacting between them in the X-ray structure. (Communicated by Masanori OTSUKA, M.J.A.)
- 日本学士院の論文
著者
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Suzuki M
National Inst. Advanced Industrial Sci. And Technol. (aist) Ibaraki Jpn
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Suzuki Masashi
National Institute Of Advanced Industrial Science And Technology
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Ishijima Sanae
National Institute of Advanced Industrial Science and Technology (AIST)
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Clowney Lester
National Institute of Advanced Industrial Science and Technology (AIST)
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Clowney Lester
National Inst. Of Advanced Industrial Sci. And Technol. (aist) Aist Tsukuba Center
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