An electron microscopic study of the archaeal feast/famine regulatory protein : 4. Estimation of the particle size
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概要
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Using the method of Fourier transform, cryo-electron micrographs of two types of archaeal feast/famine regulatory proteins (FFRPs), pot0434017 (FL11) and pot1216151 (DM1), were analyzed. After correcting the Fourier power spectra by considering effects of the contrast transfer functions (CTFs), peaks were identified at frequencies, corresponding to the particle size of ~130 Å for FL11 in the complex with DNA, in addition, a smaller size, ~40 Å for the same protein in the absence of DNA, the particle size of ~65 Å for DM1 when interacting with a ligand, and a smaller size of ~30 Å when the ligand was removed. These numbers are consistent with our previous observations that dimers of FL11 form octamers, i.e. tetrameric assemblies of the dimers, upon intercation with DNA, and that similar octamers of a smaller FFRP, DM1 of the molecular weight approximately half that of FL11, are stabilized by interaction with the ligand. Some aspects of CTF correction are discussed. (Communicated by Masanori OTSUKA, M.J.A.)
- 日本学士院の論文
著者
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Suzuki M
National Inst. Advanced Industrial Sci. And Technol. (aist) Ibaraki Jpn
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Suzuki Masashi
National Institute Of Advanced Industrial Science And Technology
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Ishijima Sanae
National Institute of Advanced Industrial Science and Technology (AIST)
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Clowney Lester
National Institute of Advanced Industrial Science and Technology (AIST)
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Clowney Lester
National Inst. Of Advanced Industrial Sci. And Technol. (aist) Aist Tsukuba Center
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