Development of Regeneration and Transformation Systems for Raphiolepis umbellata L. Plants Using Particle Bombardment
スポンサーリンク
概要
- 論文の詳細を見る
When hypocotyl sections of Raphiolepis umbellata L. were cultured in a woody plant medium containing 8.9μM benzylaminopurine and 1.1μM α-naphthaleneacetic, adventitous shoots were formed. Fully developed plants were obtained in 3 months of the culture. A chimeric plasmid pENiR harboring cDNA of Arabidopsis thaliana nitrite reductase gene (nii) under the control of an enhanced promoter consisting of sequences from both cauliflower mosaic virus 35S promoter and tobacco mosaic virus omega regions and a nopaline synthase terminator was constructed and introduced to the hypocotyl sections by particle bombardment. A plasmid pCH bearing hygromycin phosphotransferase gene (hph) was cointroduced. Transformed shoots were selected for hygromycin resistance. Polymerase chain reaction analysis showed the presence of hph gene in 16 out of 2572 bombarded hypocotyl sections, and two of which were found to bear Arabidopsis nii gene. Translation of this gene in transgenic plants was confirmed by Western blot analysis.
- 日本植物細胞分子生物学会の論文
- 2003-06-01
著者
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Sakamoto Atsushi
Department of Cardiology, Saitama Medical School
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TAKAHASHI Misa
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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MORIKAWA Hiromichi
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Takahashi M
Graduate School Of Sci. Hiroshima Univ.
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Takahashi Misa
Graduate School Of Science Hiroshima University
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森川 弘道
広島大学院・理学研究科
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Takahashi Misa
Department Of Mathematical And Life Science Graduate School Of Science Hiroshima University
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Sakamoto Atsushi
Hiroshima Univ. Higashi‐hiroshima Jpn
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Sakamoto Atsushi
Department Of Cardiology Central Hospital Of Jnr.
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Morikawa H
Graduate School Of Science Hiroshima University
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Morikawa Hiromichi
Hiroshima Univ. Higashi‐hiroshima Jpn
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Morikawa Hiromichi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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Takahashi Misa
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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ERKIN Ozgur
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Erkin Ozgur
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Morikawa Hiromichi
Grad. Dept. Gene Sci Fac. Sci. Hiroshima Univ.
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TAKAHASHI Misa
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
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