Mechanism of Transgene Integration into a Host Genome by Particle Bombardment
スポンサーリンク
概要
- 論文の詳細を見る
DNA sequences of transgenic loci in the transgenes of tobacco and Arabidopsis were analyzed. Almost all transgenic loci studied contained cis elements characteristic of nuclear matrix attachment regions (MARs). A MAR sequence isolated from one such locus of tobacco cells was cloned into the original transformation vector, and introduced into tobacco cells. The presence of the MAR sequence resulted in a five- to tenfold increase in the transformant yields, and it appeared to stimulate both the expression of the transgene and its integration into the host genome. A search using the BLAST program revealed that the transgenic loci contained junction region sequences that may have originated from different chromosomes of the nuclear genome and from the chloroplast genome.
- 日本植物細胞分子生物学会の論文
- 2002-12-01
著者
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Sakamoto Atsushi
Department of Cardiology, Saitama Medical School
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TAKAHASHI Misa
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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MORIKAWA Hiromichi
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Takahashi M
Graduate School Of Sci. Hiroshima Univ.
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Takahashi Misa
Graduate School Of Science Hiroshima University
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森川 弘道
広島大学院・理学研究科
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Takahashi Misa
Department Of Mathematical And Life Science Graduate School Of Science Hiroshima University
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Sakamoto Atsushi
Hiroshima Univ. Higashi‐hiroshima Jpn
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Sakamoto Atsushi
Department Of Cardiology Central Hospital Of Jnr.
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Morikawa H
Graduate School Of Science Hiroshima University
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Morikawa Hiromichi
Hiroshima Univ. Higashi‐hiroshima Jpn
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Morikawa Hiromichi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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Takahashi Misa
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Irifune K
Hiroshima Prefectural University School Of Bioresources
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Irifune Kohei
Grad. Dept. Gene Sci. Fac. Sci. Hiroshima Univ.
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HOKAZONO Hiroo
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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IRIFUNE Kohei
Hiroshima Prefectural University, School of Bioresources
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Hokazono Hiroo
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Morikawa Hiromichi
Grad. Dept. Gene Sci Fac. Sci. Hiroshima Univ.
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TAKAHASHI Misa
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
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