The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants
スポンサーリンク
概要
- 論文の詳細を見る
Xanthine dehydrogenase (XDH) is a ubiquitous enzyme involved in purine metabolism which catalyzes the oxidation of hypoxanthine and xanthine to uric acid. Although the essential role of XDH is well documented in the nitrogen-fixing nodules of leguminous plants, the physiological importance of this enzyme remains uncertain in non-leguminous species such as Arabidopsis. To evaluate the impact of an XDH deficiency on whole-plant physiology and development in Arabidopsis, RNA interference (RNAi) was used to generate transgenic lines of this species in which AtXDH1 and AtXDH2, the two paralogous genes for XDH in this plant, were silenced simultaneously. The nearly complete reduction in the total XDH protein levels caused by this gene silencing resulted in the dramatic overaccumulation of xanthine and a retarded growth phenotype in which fruit development and seed fertility were also affected. A less severe silencing of XDH did not cause these growth abnormalities. The impaired growth phenotype was mimicked by treating wild-type plants with the XDH inhibitor allopurinol, and was reversed in the RNAi transgenic lines by exogenous supplementation of uric acid. Inactivation of XDH is also associated with precocious senescence in mature leaves displaying accelerated chlorophyll breakdown and by the early induction of senescence-related genes and enzyme markers. In contrast, the XDH protein levels increase with the aging of the wild-type leaves, supporting the physiological relevance of the function of this enzyme in leaf senescence. Our current results thus indicate that XDH functions in various aspects of plant growth and development.
- Oxford University Pressの論文
- 2007-10-01
著者
-
Sakamoto Atsushi
Department of Cardiology, Saitama Medical School
-
TAKAHASHI Misa
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
-
MORIKAWA Hiromichi
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
-
Takahashi M
Graduate School Of Sci. Hiroshima Univ.
-
Takahashi Misa
Graduate School Of Science Hiroshima University
-
森川 弘道
広島大学院・理学研究科
-
SAKAMOTO Saori
Department of Molecular Medicinal Sciences, Graduate School of Biomedical Sciences, Nagasaki Univers
-
Takahashi Misa
Department Of Mathematical And Life Science Graduate School Of Science Hiroshima University
-
Sakamoto Atsushi
Hiroshima Univ. Higashi‐hiroshima Jpn
-
Sakamoto Atsushi
Department Of Cardiology Central Hospital Of Jnr.
-
Morikawa H
Graduate School Of Science Hiroshima University
-
Morikawa Hiromichi
Hiroshima Univ. Higashi‐hiroshima Jpn
-
Morikawa Hiromichi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
-
Takahashi Misa
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
-
Sakamoto Saori
Department Of Biological Science Faculty Of Science Hiroshima University
-
NAKAGAWA Ayami
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
-
Nakagawa Ayami
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
-
Morikawa Hiromichi
Grad. Dept. Gene Sci Fac. Sci. Hiroshima Univ.
-
TAKAHASHI Misa
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
関連論文
- Two-component high-affinity nitrate transport system in barley : Membrane localization, protein expression in roots and a direct protein-protein interaction
- OJ-001 QRS Width Does Not Reveal the Indication for Cardiac Resynchronization Therapy in Patients with Heart Failure(Arrhythmia, Non-Pharmacological Therapy 1 (A) : OJ1)(Oral Presentation (Japanese))
- OE-201 Angiotensin II Receptor 1 Blocker Improves Not Only Hypertension But Also Ventricular arrhythmogenicity(ECG/Body Surface Potential Mapping/Hotter 2 (A) : OE25)(Oral Presentation (English))
- PE-462 Initial Experience of SPECT-CT in Our hospital : Comparison with Conventional SPECT(Nuclear cardiology(05)(I),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- CHARACTERIZATION OF EXPRESSION OFGLUTAMINE SYNTHETASE GENES IN RESPONSE TO FUMIGATION WITHNO_2 IN ARABIDOPSIS THALIANA PLANTS
- Effects of ion beam irradiation on the regeneration and morphology of Ficus thunbergii Maxim
- 活性窒素代謝 : 植物窒素代謝の新しい課題
- 窒素同化とNOx代謝 (植物の代謝コミュニケーション--植物分子生理学の新展開) -- (クロストーク)
- 11-3 植物における未同定窒素化合物 (UN) 生成機構に関する研究、特に酸化ストレスとの関連について(11. 植物の栄養生態, 2006年度秋田大会講演要旨)
- Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino
- Ellagitannins and Lignan Glycosides from Balanophora japonica (Balanophoraceae)
- 植物における未同定窒素の挙動の栄養生理学的研究 : (第3報) 窒素化合形態が未同定窒素集積に与える影響(10. 植物の代謝, 2004年度大会講演要旨集)
- 10-17 植物における未同定窒素の挙動の栄養生理学的解析 : (第2報)イネ科およびマメ科植物における集積状態(10.植物の代謝)
- 10-16 植物における未同定窒素の挙動の栄養性理学的解析 : (第1報)野菜類における集積状態(10.植物の代謝)
- Differential effect of Calcium and Magnesium on Mechanical Properties of Pea Stem Cell Walls
- Oriented structure of pectic polysaccharides in pea epidermal cell walls
- Membrane Potentials of Heterotrophically Cultured Tobacco Cells
- Impact of Disease Progression on Seven-year Outcome after Multivessel Stenting in Diabetic Patients(Coronary Revascularization, PTCA/Stent/DCA/Rotablator/New Device 6 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Short and Long-Term Follow Up for Single Vessel Diabetic Patients after Stenting(Coronary Revascularization, PTCA/Stent/DCA/Rotablator/New Device 1 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Enhanced Germination under High-Salt Conditions of Seeds of Transgenic Arabidopsis with a Bacterial Gene(codA)for Choline Oxidase
- Effects of 6 Months Valsartan Therapy in Asymptomatic Patients with Chronic Severe Aortic Regurgitation(Valvular Heart Disease/Pericarditis/Cardiac Tumor 2 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- 9-53 オオムギにおける高親和性硝酸トランスポータータンパク質の発現解析(9.植物の無機栄養)
- 樹木によるファイトレメディエーション
- 1S01 ファイトレメディエーションの新展開
- ファイトレメディエーションによる環境修復の新展開 (特集 環境バイオテクノロジーの新展開)
- 植物利用による都市の再生--ファイトレメディエーションによる都市環境の修復の展望 (特集 都市と緑をめぐる新政策・新提案)
- 植物利用による大気および土壌汚染の修復
- 遺伝子工学的手法によるNO_xを好む植物の開発
- 環境汚染を食べる植物によるファイトレメディエーション
- S83 ファイトレメディエーション : 植物を利用した環境汚染の修復
- CHARACTERIZATION OF SAR SEQUENCE FLANKING ARABIDOPSIS NITRITE REDUCTASE GENE
- EFFECT OF PHYSIOLOGICAL STATE OF TARGET CELLS ON THE EXPRESSION OF TRANSGENES INTRODUCED BY PARTICLE BOMBARDMENT IN ARABIDOPSIS THALIANA
- EXPRESSION OF NITRATE REDUCTASE ANDNITRITE REDUCTASE GENES IN RESPONSE TO FUMIGATION WITHNO_2 IN ARABIDOPSIS THALINA
- CHARACTERIZATION OF TRANSGENICARABIDOPSIS THALIANA PLANTS ENRICHED WITH THE NIR GENE
- ANALYSIS OF GENES INVOLVED IN THE METABOLISM OF (NO)_2 IN ARABIDOPSIS THALIANA
- Regeneration and Transformation of a Roadside Tree Pittosporum tobira A.
- Parquet Tolerance of Transgenic Nicotiana tabacum with Enhanced Activities of Glutathione Reductase and Superoxide Dismutase : ENVIRONMENTAL AND STRESS RESPONSES
- cDNA Cloning and Expression of the Plastidic Copper/Zinc-Superoxide Dismutase from Spinach (Spinacia oleracea L.) Leaves
- STARVATION TREATMENT INCREASES EXPRESSIONEFFICIENCY OF FOREIGN GENE INTRODUCED BY PARTICLE BOMBARDMENTINTO CULTURED CELLS AND TISSUES OF PLANTS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NITROGEN DIOXIDE ABSORBED IN PLANT LEAVES
- Permeability of the Nitella internodal cell to organic substances as measured by the double chamber method
- TIMING OF THE LOCALIZATION OF p34^ IN PREPROPHASE BANDS OF ONION ROOT TIPS
- DETERMINATION OF LEVELS OF NO_3^-, NH_2 AND NO_4^+ IONS IN LEAVES OF VARIOUS PLANTS BY CAPILLARY ELECTROPHORESIS
- Determination of Levels of NO_3^-,NO_2^- and NH_4^+ Ions in Leaves of Various Plants by Capillary Electrophoresis
- PJ-367 Effectiveness of Sirolimus-eluting Stent for Diffuse In-stent Restenosis(Coronary revascularization, PCI-17 (IHD) PJ62,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- ANALYSIS OF THE METABOLIC PATHWAY OF NITROUS OXIDE DERIVED FROM TOBACCO INTRODUCED NiR ANTISENSE cDNA
- MASS SPECTROMETRIC ANALYSIS OF N_2O GAS DERIVED FROM TOBACCO INTRODUCED NiR ANTISENSE cDNA
- REDUCTION OF NITRATE AND NITRITE IN TRANSGENIC PLANTS THAT HAVE HIGHLY REDUCED NITRITE REDUCTASE ACTIVITY (3) ANALYSIS OF PROTEINS INVOLVED
- REDUCTION OF NITRATE IN PLANTS THAT LACK NITRITE REDUCTASE ACTIVITY
- The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants
- Phytoremediators from abandoned rice field
- IJ1 is an orientation-independent transformation enhancer sequence
- Development of Regeneration and Transformation Systems for Raphiolepis umbellata L. Plants Using Particle Bombardment
- Mechanism of Transgene Integration into a Host Genome by Particle Bombardment
- ファイトレメディエーション
- ファイトレメディエーションの新展開 (特集 地球環境とバイオテクノロジー) -- (第2部 環境バイオテクノロジー)
- ファイトレメディエーションにおける農業技術の重要性(2)
- Oriented structure of matrix polysaccharides in and extension growth of Nitella cell wall
- STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE JUNCTION REGIONS BETWEEN TOBACCO GENOME AND TRANSGENE INTRODUCED BY PARTICLE BOMBARDMENT.
- SAR-LIKE SEQUENCES DERIVED FROM JUNCTIONREGION IN TRANSGENIC TOBACCO CELLS INCREASES TRANSFORMATIONFREQUENCIES IN BOMBARDMENT-MEDIATED TRANSFORMATION
- DETERMINATION OF JUNCTION SEQUENCES INTRANSGENIC TOBACCO CELLS OBTAINED BY PARTICLE BOMBARDMENT
- COMPARISON BETWEEN ECG-GATED SCINTIANGIOCARDIOGRAPHY AND CONTRAST ANGIOCARDIOGRAPHY IN MEASURING LEFT VENTRICULAR VOLUME AND EJECTION FRACTION
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN (UN) DERIVED FROM NITROGEN DIOXIDE ABSORBED IN PLANT LEAVES
- METABOLISM OF NITROGEN OXIDES IN PLANTS-FORMATION OF UN COMPOUNDS AND N_2O
- ASSIMILATION OF NITROGEN DIOXIDE IN TRANSGENIC PLANTS (3) NR AND GS EXPRESSORS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NO_2 ABSORBED IN PLANT LEAVES (3) PURIFICATION AND ANALYSIS OF MAIN COMPOUNDS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NO_2 ABSORBED IN PLANT LEAVES
- PROTEINS EXPRESSED IN RESPONSE TO FUMIGATION WITH NITROGEN DIOXIDE IN PLANT LEA VES
- EMISSION OF N_2O FROM HIGHER PLANTS (2) ROLE OF PHOTOSYNTHETIC ELECTRON FLOW
- Expression of the β-Glucuronidase Gene Introduced into Intact Leaves Attached to Arabidopsis thaliana Plants by Particle Gun
- CHARACTERIZATION OF NO_2 ASSIMILATION ABILITY OF TRANSGENIC ARABIDOPSIS THALIANA PLANTS ENRICHED WITH THE NIR GENE
- DIAGNOSIS OF MYOCARDIAL ISCHEMIA MASKED BY PRIMARY AND SECONDARY ST・T CHANGES USING ^Tl MYOCARDIAL SCINTIGRAPHY : Nuclear Cardiology : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- STUDY TO MAKE CLEAR DEMONSTRATION OF MYOCARDIAL IMAGE OF THE PATIENT WITH MYOCARDIAL INFARCTION AND COMPARISON WITH THE FINDING OF ECG : IVth Auditorium
- Infrared analysis of oat coleoptile cell walls and oriented structure of matrix polysaccharides in the walls
- Influence of Axis Removal on the Expression of a Gene for a Cysteine Endopeptidase (EP-C1) in French Bean Cotyledons during Germination : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
- On the mechanism of nitrogen dioxide assimilation in Arabidopsis thaliana leaves
- IN PLANTA TRANSIENT EXPRESSION ASSAY FOR THE EFFECT OF ANTISENSE GS cDNA INTRODUCED INTO ARABIDOPSIS THALIANA LEAVES BY PARTICLE BOMBARDMENT
- CLONING OF EARLY RESPONSE GENES TONO_2 FUMIGATION IN ARABIDOPSIS THALIANA
- Expression Pattern of Bacterial Polycistronic Genes in Tobacco Cells
- RENIN RELEASE AND RENAL SYMPATHETIC EFFERENT DISCHARGE FOLLOWING VERTEBRAL ARTERY EMBOLISM
- CHARACTERIZATION OF THE EFFECT OF INTRODUCTION OF SENSE GS cDNA INTO THE LEAVES OF ARABIDOPSIS THALIANA BY PARTICLE BOMBARDMENT
- ファイトレメディエーションにおける農業技術の重要性(1)
- Visual Acuity Following Intravitreal Bevacizumab for Macular Edema Associated with Retinal Vein Occlusion
- CHARACTERIZATION OF TRANSGENIC ARABIDOPSIS THALIANA PLANTS ENRICHED WITH THE NIR GENE
- Effect of auxin on changes in the oriented structure of wall polysaccharides in response to mechanical extension in oat coleoptile cell walls
- Infrared analysis of pea stem cell walls and oriented structure of matrix polysaccharides in them
- Metabolic fate of the nitrogen of nitrogen dioxide gas taken into plant leaves
- A NOVEL PARTICLE GUN DEVICE DRIVEN BY HAMMER SHOOK -DESIGN OF THE DEVICE AND GENE EXPRESSION INTRODUCED BY IT
- STUDY ON PLANT ENZYMES THAT PRTICIPATE IN DEGRADATION OF AROMATIC COMPOUNDS IN THE RHIZOSPHERE
- Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants
- Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants
- Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants
- ファイトレメディエーションとファイトテクノロジー--米国におけるプライベートセクターの活動と大気汚染修復壁面緑化 (特集 環境バイオテクノロジーの事業化)
- Ventricular Tachycardia on Previous Myocardial Infarction: Effective by Nifekalant, Bepridil and Two Sessions of Catheter Ablation
- A Case of Ventricular Tachycardia with Dilated Cardiomyopathy Controlled by D-Sotalol and Catheter Ablation
- Left Ventricular Improvement after Cardiac Resynchronization Therapy among Ischemic, Non-Ischemic and Right Ventricular Pacing-Induced Cardiomyopathy
- Endless loop tachycardia below the upper tracking rate of a pacemaker: A case report
- Synthesis of the cyclotetrasilanes of the type((PhRSi)4) (R=t-Bu ; BuCH2).
- In Vitro Attachment of Bovine Hatched Blastocysts on Fibronectin is Mediated by Integrin in a RGD Dependent Manner