IJ1 is an orientation-independent transformation enhancer sequence
スポンサーリンク
概要
- 論文の詳細を見る
A 507-bp nuclear matrix attachment region (MAR) sequence (designated TJ1) was flanked at both at 5′ and 3′ ends of the expression cassette for a modified green fluorescent protein (GFP) gene of the 35 Ω-sGFP(S65T) in four different orientations. A plasmid p507TH bearing a 507-bp non-MAR sequence from yeast at both ends of the cassette also was constructed. These five plasmids and 35 Ω-sGFP(S65T) were separately delivered into cultured tobacco BY-2 cells by particle bombardment. The yield of stable transformants that showed a uniform green fluorescence was determined. Southern blot analysis confirmed the integration of the GFP gene in tobacco genome. Transformant yields of the two TJ1-lacking plasmids were not significant. Transformant yields appeared to be increased by more than 2 times by the presence of TJ1-MAR sequences. The orientation of TJ1-MAR sequences did not influence the transformant yield.
- 日本植物細胞分子生物学会の論文
- 2005-06-01
著者
-
Sakamoto Atsushi
Department of Cardiology, Saitama Medical School
-
TAKAHASHI Misa
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
-
MORIKAWA Hiromichi
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
-
Takahashi M
Graduate School Of Sci. Hiroshima Univ.
-
Takahashi Misa
Graduate School Of Science Hiroshima University
-
森川 弘道
広島大学院・理学研究科
-
Takahashi Misa
Department Of Mathematical And Life Science Graduate School Of Science Hiroshima University
-
Sakamoto Atsushi
Hiroshima Univ. Higashi‐hiroshima Jpn
-
Sakamoto Atsushi
Department Of Cardiology Central Hospital Of Jnr.
-
Morikawa H
Graduate School Of Science Hiroshima University
-
Morikawa Hiromichi
Hiroshima Univ. Higashi‐hiroshima Jpn
-
Morikawa Hiromichi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
-
Takahashi Misa
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
-
HOKAZONO Hiroo
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
-
Hokazono Hiroo
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
-
Morikawa Hiromichi
Grad. Dept. Gene Sci Fac. Sci. Hiroshima Univ.
-
TAKAHASHI Misa
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
関連論文
- Two-component high-affinity nitrate transport system in barley : Membrane localization, protein expression in roots and a direct protein-protein interaction
- OJ-001 QRS Width Does Not Reveal the Indication for Cardiac Resynchronization Therapy in Patients with Heart Failure(Arrhythmia, Non-Pharmacological Therapy 1 (A) : OJ1)(Oral Presentation (Japanese))
- OE-201 Angiotensin II Receptor 1 Blocker Improves Not Only Hypertension But Also Ventricular arrhythmogenicity(ECG/Body Surface Potential Mapping/Hotter 2 (A) : OE25)(Oral Presentation (English))
- PE-462 Initial Experience of SPECT-CT in Our hospital : Comparison with Conventional SPECT(Nuclear cardiology(05)(I),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- CHARACTERIZATION OF EXPRESSION OFGLUTAMINE SYNTHETASE GENES IN RESPONSE TO FUMIGATION WITHNO_2 IN ARABIDOPSIS THALIANA PLANTS
- Effects of ion beam irradiation on the regeneration and morphology of Ficus thunbergii Maxim
- 活性窒素代謝 : 植物窒素代謝の新しい課題
- 窒素同化とNOx代謝 (植物の代謝コミュニケーション--植物分子生理学の新展開) -- (クロストーク)
- 11-3 植物における未同定窒素化合物 (UN) 生成機構に関する研究、特に酸化ストレスとの関連について(11. 植物の栄養生態, 2006年度秋田大会講演要旨)
- Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino
- 植物における未同定窒素の挙動の栄養生理学的研究 : (第3報) 窒素化合形態が未同定窒素集積に与える影響(10. 植物の代謝, 2004年度大会講演要旨集)
- 10-17 植物における未同定窒素の挙動の栄養生理学的解析 : (第2報)イネ科およびマメ科植物における集積状態(10.植物の代謝)
- 10-16 植物における未同定窒素の挙動の栄養性理学的解析 : (第1報)野菜類における集積状態(10.植物の代謝)
- Differential effect of Calcium and Magnesium on Mechanical Properties of Pea Stem Cell Walls
- Oriented structure of pectic polysaccharides in pea epidermal cell walls
- Membrane Potentials of Heterotrophically Cultured Tobacco Cells
- Impact of Disease Progression on Seven-year Outcome after Multivessel Stenting in Diabetic Patients(Coronary Revascularization, PTCA/Stent/DCA/Rotablator/New Device 6 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Short and Long-Term Follow Up for Single Vessel Diabetic Patients after Stenting(Coronary Revascularization, PTCA/Stent/DCA/Rotablator/New Device 1 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Enhanced Germination under High-Salt Conditions of Seeds of Transgenic Arabidopsis with a Bacterial Gene(codA)for Choline Oxidase
- Effects of 6 Months Valsartan Therapy in Asymptomatic Patients with Chronic Severe Aortic Regurgitation(Valvular Heart Disease/Pericarditis/Cardiac Tumor 2 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- 9-53 オオムギにおける高親和性硝酸トランスポータータンパク質の発現解析(9.植物の無機栄養)
- 樹木によるファイトレメディエーション
- 1S01 ファイトレメディエーションの新展開
- ファイトレメディエーションによる環境修復の新展開 (特集 環境バイオテクノロジーの新展開)
- 植物利用による都市の再生--ファイトレメディエーションによる都市環境の修復の展望 (特集 都市と緑をめぐる新政策・新提案)
- 植物利用による大気および土壌汚染の修復
- 遺伝子工学的手法によるNO_xを好む植物の開発
- 環境汚染を食べる植物によるファイトレメディエーション
- S83 ファイトレメディエーション : 植物を利用した環境汚染の修復
- CHARACTERIZATION OF SAR SEQUENCE FLANKING ARABIDOPSIS NITRITE REDUCTASE GENE
- EFFECT OF PHYSIOLOGICAL STATE OF TARGET CELLS ON THE EXPRESSION OF TRANSGENES INTRODUCED BY PARTICLE BOMBARDMENT IN ARABIDOPSIS THALIANA
- EXPRESSION OF NITRATE REDUCTASE ANDNITRITE REDUCTASE GENES IN RESPONSE TO FUMIGATION WITHNO_2 IN ARABIDOPSIS THALINA
- CHARACTERIZATION OF TRANSGENICARABIDOPSIS THALIANA PLANTS ENRICHED WITH THE NIR GENE
- ANALYSIS OF GENES INVOLVED IN THE METABOLISM OF (NO)_2 IN ARABIDOPSIS THALIANA
- Regeneration and Transformation of a Roadside Tree Pittosporum tobira A.
- Parquet Tolerance of Transgenic Nicotiana tabacum with Enhanced Activities of Glutathione Reductase and Superoxide Dismutase : ENVIRONMENTAL AND STRESS RESPONSES
- cDNA Cloning and Expression of the Plastidic Copper/Zinc-Superoxide Dismutase from Spinach (Spinacia oleracea L.) Leaves
- STARVATION TREATMENT INCREASES EXPRESSIONEFFICIENCY OF FOREIGN GENE INTRODUCED BY PARTICLE BOMBARDMENTINTO CULTURED CELLS AND TISSUES OF PLANTS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NITROGEN DIOXIDE ABSORBED IN PLANT LEAVES
- Permeability of the Nitella internodal cell to organic substances as measured by the double chamber method
- TIMING OF THE LOCALIZATION OF p34^ IN PREPROPHASE BANDS OF ONION ROOT TIPS
- DETERMINATION OF LEVELS OF NO_3^-, NH_2 AND NO_4^+ IONS IN LEAVES OF VARIOUS PLANTS BY CAPILLARY ELECTROPHORESIS
- Determination of Levels of NO_3^-,NO_2^- and NH_4^+ Ions in Leaves of Various Plants by Capillary Electrophoresis
- PJ-367 Effectiveness of Sirolimus-eluting Stent for Diffuse In-stent Restenosis(Coronary revascularization, PCI-17 (IHD) PJ62,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- ANALYSIS OF THE METABOLIC PATHWAY OF NITROUS OXIDE DERIVED FROM TOBACCO INTRODUCED NiR ANTISENSE cDNA
- MASS SPECTROMETRIC ANALYSIS OF N_2O GAS DERIVED FROM TOBACCO INTRODUCED NiR ANTISENSE cDNA
- REDUCTION OF NITRATE AND NITRITE IN TRANSGENIC PLANTS THAT HAVE HIGHLY REDUCED NITRITE REDUCTASE ACTIVITY (3) ANALYSIS OF PROTEINS INVOLVED
- REDUCTION OF NITRATE IN PLANTS THAT LACK NITRITE REDUCTASE ACTIVITY
- The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants
- Phytoremediators from abandoned rice field
- IJ1 is an orientation-independent transformation enhancer sequence
- Development of Regeneration and Transformation Systems for Raphiolepis umbellata L. Plants Using Particle Bombardment
- Mechanism of Transgene Integration into a Host Genome by Particle Bombardment
- ファイトレメディエーション
- ファイトレメディエーションの新展開 (特集 地球環境とバイオテクノロジー) -- (第2部 環境バイオテクノロジー)
- ファイトレメディエーションにおける農業技術の重要性(2)
- Oriented structure of matrix polysaccharides in and extension growth of Nitella cell wall
- STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE JUNCTION REGIONS BETWEEN TOBACCO GENOME AND TRANSGENE INTRODUCED BY PARTICLE BOMBARDMENT.
- SAR-LIKE SEQUENCES DERIVED FROM JUNCTIONREGION IN TRANSGENIC TOBACCO CELLS INCREASES TRANSFORMATIONFREQUENCIES IN BOMBARDMENT-MEDIATED TRANSFORMATION
- DETERMINATION OF JUNCTION SEQUENCES INTRANSGENIC TOBACCO CELLS OBTAINED BY PARTICLE BOMBARDMENT
- COMPARISON BETWEEN ECG-GATED SCINTIANGIOCARDIOGRAPHY AND CONTRAST ANGIOCARDIOGRAPHY IN MEASURING LEFT VENTRICULAR VOLUME AND EJECTION FRACTION
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN (UN) DERIVED FROM NITROGEN DIOXIDE ABSORBED IN PLANT LEAVES
- METABOLISM OF NITROGEN OXIDES IN PLANTS-FORMATION OF UN COMPOUNDS AND N_2O
- ASSIMILATION OF NITROGEN DIOXIDE IN TRANSGENIC PLANTS (3) NR AND GS EXPRESSORS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NO_2 ABSORBED IN PLANT LEAVES (3) PURIFICATION AND ANALYSIS OF MAIN COMPOUNDS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NO_2 ABSORBED IN PLANT LEAVES
- PROTEINS EXPRESSED IN RESPONSE TO FUMIGATION WITH NITROGEN DIOXIDE IN PLANT LEA VES
- EMISSION OF N_2O FROM HIGHER PLANTS (2) ROLE OF PHOTOSYNTHETIC ELECTRON FLOW
- Expression of the β-Glucuronidase Gene Introduced into Intact Leaves Attached to Arabidopsis thaliana Plants by Particle Gun
- CHARACTERIZATION OF NO_2 ASSIMILATION ABILITY OF TRANSGENIC ARABIDOPSIS THALIANA PLANTS ENRICHED WITH THE NIR GENE
- DIAGNOSIS OF MYOCARDIAL ISCHEMIA MASKED BY PRIMARY AND SECONDARY ST・T CHANGES USING ^Tl MYOCARDIAL SCINTIGRAPHY : Nuclear Cardiology : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- STUDY TO MAKE CLEAR DEMONSTRATION OF MYOCARDIAL IMAGE OF THE PATIENT WITH MYOCARDIAL INFARCTION AND COMPARISON WITH THE FINDING OF ECG : IVth Auditorium
- Infrared analysis of oat coleoptile cell walls and oriented structure of matrix polysaccharides in the walls
- Influence of Axis Removal on the Expression of a Gene for a Cysteine Endopeptidase (EP-C1) in French Bean Cotyledons during Germination : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
- On the mechanism of nitrogen dioxide assimilation in Arabidopsis thaliana leaves
- IN PLANTA TRANSIENT EXPRESSION ASSAY FOR THE EFFECT OF ANTISENSE GS cDNA INTRODUCED INTO ARABIDOPSIS THALIANA LEAVES BY PARTICLE BOMBARDMENT
- CLONING OF EARLY RESPONSE GENES TONO_2 FUMIGATION IN ARABIDOPSIS THALIANA
- Expression Pattern of Bacterial Polycistronic Genes in Tobacco Cells
- RENIN RELEASE AND RENAL SYMPATHETIC EFFERENT DISCHARGE FOLLOWING VERTEBRAL ARTERY EMBOLISM
- CHARACTERIZATION OF THE EFFECT OF INTRODUCTION OF SENSE GS cDNA INTO THE LEAVES OF ARABIDOPSIS THALIANA BY PARTICLE BOMBARDMENT
- ファイトレメディエーションにおける農業技術の重要性(1)
- Visual Acuity Following Intravitreal Bevacizumab for Macular Edema Associated with Retinal Vein Occlusion
- CHARACTERIZATION OF TRANSGENIC ARABIDOPSIS THALIANA PLANTS ENRICHED WITH THE NIR GENE
- Effect of auxin on changes in the oriented structure of wall polysaccharides in response to mechanical extension in oat coleoptile cell walls
- Infrared analysis of pea stem cell walls and oriented structure of matrix polysaccharides in them
- Metabolic fate of the nitrogen of nitrogen dioxide gas taken into plant leaves
- A NOVEL PARTICLE GUN DEVICE DRIVEN BY HAMMER SHOOK -DESIGN OF THE DEVICE AND GENE EXPRESSION INTRODUCED BY IT
- STUDY ON PLANT ENZYMES THAT PRTICIPATE IN DEGRADATION OF AROMATIC COMPOUNDS IN THE RHIZOSPHERE
- Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants
- Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants
- Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants
- ファイトレメディエーションとファイトテクノロジー--米国におけるプライベートセクターの活動と大気汚染修復壁面緑化 (特集 環境バイオテクノロジーの事業化)
- Ventricular Tachycardia on Previous Myocardial Infarction: Effective by Nifekalant, Bepridil and Two Sessions of Catheter Ablation
- A Case of Ventricular Tachycardia with Dilated Cardiomyopathy Controlled by D-Sotalol and Catheter Ablation
- Left Ventricular Improvement after Cardiac Resynchronization Therapy among Ischemic, Non-Ischemic and Right Ventricular Pacing-Induced Cardiomyopathy
- Endless loop tachycardia below the upper tracking rate of a pacemaker: A case report
- Synthesis of the cyclotetrasilanes of the type((PhRSi)4) (R=t-Bu ; BuCH2).
- In Vitro Attachment of Bovine Hatched Blastocysts on Fibronectin is Mediated by Integrin in a RGD Dependent Manner