Cloning of the S_8-RNase (S_8-allele) of Japanese Pear (Pyrus pyrifolia Nakai)

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概要

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• The expression of S8-RNase was confirmed in pistils of two Japanese pear cultivars, ‘Ichiharawase’ (S1S8) and ‘Heiwa’ (S4S8). The complete sequence of the S8-RNase gene was determined connecting the nucleotide sequences of partial cDNA and 5 terminal genomic DNA fragments amplified by RT-PCR and genomic PCR. The S8-RNase has an open reading frame of 684 nucleotides encoding 228 amino acid residues. A hypervariable region (HV) of S8-RNase, which is quite different from those of S1- to S7-RNases, includes an intron of 234 bp. The similarity of deduced amino acid sequences between S8-RNase and the seven S-RNases of Japanese pear ranged from 56.7% (S3-RNase) to 70.2% (S7-RNase). Based on its nucleotide sequence, we selected NruI as S8-RNase specific restriction endonuclease and established the PCR-RFLP system for discriminating S1- to S8-alleles.

• 日本植物細胞分子生物学会の論文
• 2002-03-01

著者

• Castillo Carlos

  神戸大院自然科学研究科

• Natsuka Shunji

  Department Of Chemistry Osaka University Graduate School Of Science

• Norioka Shigemi

  Institute For Protein Research Osaka University

• Norioka Shigemi

  The Institute For Protein Research Osaka University

• Norioka Shigemi

  Graduate School Of Frontierbiosciences Osaka University

• Sakamoto Tomoaki

  National Institute Of Agrobiological Sciences:(present Address)field Production Science Center Gradu

• Takasaki Takeshi

  Faculty Of Agriculture Kobe University

• Castillo Carlos

  Graduate School Of Science And Technology Kobe University

• Nakanishi Tetsu

  Graduate School Of Science And Technology Kobe University

• Nakanishi Tetsu

  Graduate School Of Agricultural Science Kobe University

• Norioka S

  Osaka Univ. Osaka Jpn

• Norioka S

  The Institute For Protein Research Osaka University

• Saito T

  National Institute Of Fruit Tree Science

• Saito Toshihiro

  National Institute of Fruit Tree Science

• Takasaki Takeshi

  n

• Nakanishi Tetsu

  Institute for Protein Research, Osaka University

• Takasaki T

  Kobe Univ. Kobe Jpn

• Castillo Carlos

  Graduate School Of Agricultural Science Kobe University

• Sakamoto Tomoaki

  Inst. Agr. & Forest. Univ. Tsukuba

• Nakanishi T

  Kobe Univ. Kobe Jpn

• Takasaki T

  Kobe Univ. Kobe

• Saito Toshihiro

  National Institute of Agrobiological Sciences:(Present address)Field Production Science Center, Graduate School of Agricultural and Life Sciences, University of Tokyo

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