S-genotype Assignments of Local Cultivars in Japanese Pear 'Senryo', 'Kuroki', and 'Hogyoku'

スポンサーリンク

概要

• 論文の詳細を見る

• Japanese pear exhibits gametophytic self-incompatibility controlled by a single S-locus with multiple alleles. The S-locus encodes an S-RNase as a stylar product, and 10 S-RNase alleles (S_1 to S_9, and S_k) have been cloned from major cultivars. To investigate the diversity of S-alleles in Japanese pear, we analyzed the S-genotypes of three local cultivars ('Senryo', 'Kuroki', and 'Hogyoku'). Two S-RNase fragments were amplified from each cultivar by genomic PCR with S-RNase-specific primers. A cleaved amplified polymorphic sequence (CAPS) marker system to distinguish S_1- to S_9-RNases assigned S_3- and S_2-RNase alleles to 'Senryo' and 'Kuroki', respectively. Cloning and sequencing of the other S-RNase genes identified S_e-RNase of European pear in 'Senryo', S_<12>-RNase of Chinese pear in 'Kuroki', and S_<30>-RNase of Chinese pear and S_k-RNase in 'Hogyoku'. Therefore, S-genotypes of 'Senryo', 'Kuroki', and 'Hogyoku' were assigned as S_3S_e, S_2S_<12>, and S_kS_<30>, respectively. These results revealed that Japanese pear has some of the same S-alleles as European and Chinese pears as well as S_1- to S_9- and S_k-alleles.

• 園芸学会の論文

著者

• OKADA Kazuma

  Graduate School of Science & Technology, Kyoto Institute of Technology

• Takasaki-yasuda Takeshi

  Graduate School Of Agricultural Science Kobe University

• Castillo Carlos

  Graduate School Of Science And Technology Kobe University

• Okada Kazuma

  Graduate School Of Agricultural Science Kobe University

• Nakanishi Tetsu

  Graduate School Of Science And Technology Kobe University

• Nakanishi Tetsu

  Graduate School Of Agricultural Science Kobe University

• Sawamura Yutaka

  National Institute of Fruit Tree Science

• Castillo Carlos

  Graduate School Of Agricultural Science Kobe University

関連論文

• スギ雄花分化時期と気象要因の検討
• A Novel Chip Device Based on Wired Capillary Packed with High Performance Polymer-based Monolith for HPLC : Reproducibility in Preparation Processes to Obtain Long Columns
• ニホンナシ'雲井'のS遺伝子型の再検討
• ニホンナシ品種'雲井'と'世界一'のS遺伝子型はS1S3である
• ニホンナシ品種のS遺伝子型を推定するPCR-RFLPシステム(S_1-S_9)の開発
• ニホンナシ品種'天の川', '新高', '新興', '新星', '越後', '南水'と'南月'の S_9-RNase の解析
• Identification of Parent-offspring Relationships in 55 Japanese Pear Cultivars Using S-RNase Allele and SSR markers
• Integrated Reference Genetic Linkage Maps of Pear Based on SSR and AFLP Markers
• Cloning of the S_8-RNase (S_8-allele) of Japanese Pear (Pyrus pyrifolia Nakai)
• Reconsideration of S-genotype Assignments, and Discovery of a New Allele Based on S-RNase PCR-RFLPs in Japanese Pear Cultivars
• PCR-RFLPに基づくニホンナシ品種S遺伝子型の再考と新規S遺伝子の同定
• PCR-RFLPによるニホンナシのS遺伝子型の同定 : II. 新規S_8-RNase (S_8-allele) の解析と丹沢など3品種のS遺伝子型の確認
• PCR-RFLPによるニホンナシのS遺伝子型の同定
• S-genotype Assignments of Local Cultivars in Japanese Pear 'Senryo', 'Kuroki', and 'Hogyoku'
• Development of novel EST-SSR markers derived from Japanese pear (Pyrus pyrifolia)
• Genetic diversity of Japanese chestnut cultivars assessed by SSR markers
• Development of cultivar-specific DNA markers based on retrotransposon-based insertional polymorphism in Japanese pear
• Potential assessment of genome-wide association study and genomic selection in Japanese pear Pyrus pyrifolia

もっと見る 閉じる

スポンサーリンク