The N-Terminal Sequence of Paratropomyosin Binding Fragments from β-Connectin(Biochemistry & Molecular Biology)

スポンサーリンク

概要

• 論文の詳細を見る

• In order to clarify the position where paratropomyosin binds to connectin in the A-I junction region of a sarcomere, chicken β-connectin was digested by Staphylococcus aureus V8 protease under denaturing conditions and the digested peptides were electrophoretically separated. Five peptides, 150-kDa, 100-kDa, 70-kDa, and 43-kDa fragments, were simultaneously detected by biotinylated paratropomyosin and an anticonnectin monoclonal antibody. The N-terminal sequence of the 43-kDa fragment was found to be YQFRVYAVNK, similar to the sequence of 7556-7565 amino acids in the 151 fibronectin type 3 domain that was located at the A-I junction region of human cardiac titin/connectin. Therefore, we propose that paratropomyosin binds to the 43-kDa fragment from β-connectin at the A-I junction region in both living muscle and in muscle immediately postmortem, and the N-terminus of the 43-kDa fragment is localized in the 151 domain.

• 社団法人日本農芸化学会の論文
• 2003-03-23

著者

• Yoshimura Yumi

  Institute For Protein Research Osaka University

• Norioka Shigemi

  Institute For Protein Research Osaka University

• Ueda Shuji

  Graduate School Of Science And Technology Kobe University:(present Address)department Of Molecular A

• YAMANOUE Minoru

  Faculty of Agriculture, Kobe University

• OHASHI Akiko

  Faculty of Agriculture, Kobe University

• Ohashi Akiko

  The Department Of Pathology Sumitomo Hospital

• Ohashi Akiko

  Faculty Of Agriculture Kobe University

• Yamanoue Minoru

  Faculty Of Agriculture Kobe University

• Ohashi A

  Faculty Of Agriculture Kobe University

関連論文

• 試料調製法と分析技術 : 1. 2 主な分析技術
• PCR法による食肉・肉製品の肉種鑑別
• 鶏筋原線維蛋白質の可溶化に及ぼすカルシウムイオンの影響
• 豚骨格筋からのパラトロポミオシンの調製に関する研究 : 屠殺直後肉と凍結貯蔵肉の比較
• 神戸大学農学部生物機能化学科動物資源利用化学教室における研究成果
• The N-Terminal Sequence of Paratropomyosin Binding Fragments from β-Connectin(Biochemistry & Molecular Biology)
• Cloning of the S_8-RNase (S_8-allele) of Japanese Pear (Pyrus pyrifolia Nakai)
• Reconsideration of S-genotype Assignments, and Discovery of a New Allele Based on S-RNase PCR-RFLPs in Japanese Pear Cultivars
• Cushing's Syndrome with a Large Pituitary Adenoma Producing boty Corticotropin-releasing Hormone (CRH) and Adrenocorticotropin (ACTH)
• A Natural History of Adrenocorticotropin-Independent Bilateral Adrenal Macronodular Hyperplasia (AIMAH) from Preclinical to Clinically Overt Cushing's Syndrome
• Myofibrillar Structural Weakening and Tenderization of Uncooked Cold Shortened Bovine Muscle during Postmortem Storage
• 熟成中の食肉軟化を誘起する筋原線維タンパク質パラトロポミオシンの局在と遊離について
• Interaction of Paratropomyosin with β-Connectin and Its 400-kiloDalton Fragment from Chicken Skeletal Muscle as Influenced by the Calcium Ion Concentration
• Binding of Paratropomyosin to β-Connectin from Chicken Skeletal Muscle
• 熟成中の鶏,兎および豚骨格筋における筋原線維構造の脆弱化および軟らかさの畜種別比較

もっと見る 閉じる

スポンサーリンク