An Efficient and Reproducible in vitro Plant Regeneration from Leaf Discs in Pear Cultivars (Pyrus spp.)
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概要
- 論文の詳細を見る
Leaves derived from in vitro-grown apical buds were used to explore the conditions required for induction of adventitious buds and regeneration of shoots. High efficiency of shoot regeneration was obtained with half-strength MS medium (1/2MS) for Pyrus pyrifolia, and Nitsch-Nitsch (NN) medium for Pyrus communis cultivars. In both species, the basal region of the leaf was more suitable than the distal region for callus and shoot bud formation. High concentration (5 mg l-1) of thidiazuron efficiently induced callus formation and shoot bud initiation in both species. The size, colour and shape of calli were the important factors for successful shoot regeneration. Higher frequencies of callus production and rooting from regenerated shoots were observed with increasing indolebutyric acid concentration in rooting medium. However, there was a significant genotypic difference in adventitious shoot and root regeneration of each pear cultivar.
- 日本植物細胞分子生物学会の論文
- 2003-12-01
著者
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HENNAYAKE Chandani
Graduate School of Science and Technology, Kobe University
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Takasaki Takeshi
Faculty Of Agriculture Kobe University
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Hennayake Chandani
Graduate School Of Science And Technology Kobe University
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Nakanishi Tetsu
Faculty Of Agriculture Tohoku University:(present) Faculty Of Agriculture Kobe University
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Nakanishi Tetsu
Faculty Of Agriculture Kobe University
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DISSANAYAKE Kumara
Graduate School of Science and Technology, Kobe University
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MATSUDA Narumi
Horticultural Experiment Station of Yamagata Prefecture
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Dissanayake Kumara
Graduate School Of Science And Technology Kobe University
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TAKASAKI Takeshi
Faculty of Agriculture, Kobe University
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