Single nucleotide polymorphisms in an STS region linked to the Ncc-tmp1A locus are informative for characterizing the differentiation of chromosome 1A in wheat

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概要

• 論文の詳細を見る

• Homoeoalleles of Ncc confer nucleus-cytoplasm (NC) compatibility on NC hybrids of wheat with the D plasmon of Aegilops squarrosa. To dissect the chromosomal region containing Ncc, a RAPD marker linked to the Ncc-tmp1A locus, which is located on chromosome 1A of T. timopheevi, was sequenced and converted to a PCR-based sequence-tagged-site (STS) marker. Five single nucleotide polymorphisms (SNPs) between T. timopheevi and T. turgidum. were detected in a 509-bp genomic DNA fragment. Based on the SNPs, the STS alleles in 164 accessions from emmer wheat, timopheevi wheat and two einkorn wheats, T. urartu and T. boeoticum were surveyed by PCR-RFLP analysis. The sequence comparisons and PCR-RFLP analyses revealed nine alleles based on six SNPs. These SNPs were highly conserved within each group of wheat, and all groups could be distinguished by particular combinations of the SNPs. All accessions of T. urartu had one unique STS allele as compared with the others. Our results indicate that the SNPs in the STS marker linked to the Ncc-tmp1A locus would be informative for studies of the differentiation of chromosome 1A in wheat.

• 日本遺伝学会の論文

著者

• Mori N

  Lab. Of Plant Genetics Graduate School Of Agricultural Sci. Kobe Univ.

• Mori Naoki

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• Mori N

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• MORI Naoki

  Laboratory of Genetics, Faculty of Agriculture, Kobe University

• NAKAMURA Chiharu

  Laboratory of Plant Genetics, Faculty of Agriculture, Kobe University

• ASAKURA Nobuaki

  Laboratory of Biology, Faculty of Engineering, Kanagawa University

• OHTSUKA Ichiro

  Laboratory of Biology, Faculty of Engineering, Kanagawa University

• ISHIDO Takao

  Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agricul

• Mori Naoki

  神戸大学農学部

• Nakamura C

  Lab. Of Plant Genetics Dep. Of Agroenvironmental Sci. Graduate School Of Agricultural Sci. Kobe Univ

• Mori N

  Laboratory Of Plant Genetics Department Of Biological And Environmental Science Graduate School Of A

• Ishido Takao

  Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu

• Ohtsuka Ichiro

  Laboratory Of Biology Faculty Of Engineering Kanagawa University

• Nakamura Chiharu

  Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu

• Mori Naoki

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Nakamura Chiharu

  Laboratory Of Plant Genetics Department Of Agroenvironmental Science Graduate School Of Agricultural

• Mori Naoki

  Laboratory Of Chemical Ecology Graduate School Of Agriculture Kyoto University

• Asakura Nobuaki

  Laboratory Of Biology Faculty Of Engineering Kanagawa University

• Mori Naoki

  Laboratory Of Plant Genetics Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University

• NAKAMURA Chiharu

  Laboratory of Genetics, Faculty of Agriculture, Kobe University

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