Genotyping of the Q locus in wheat by a simple PCR-RFLP method

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概要

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• The Q locus located on the long arm of chromosome 5A is a key factor in evolution and widespread cultivation of domesticated wheat. The Q locus pleiotropically affects many agronomically important traits including threshability, glume shape and tenacity, rachis fragility and others. Genotyping of the Q locus based on the complex traits is ambiguous due to their multi-genetic control through interactions with the Q locus. To determine the Q locus genotype of wheat accessions possessing A genome, we developed a method based on polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) analysis. The Q and q alleles were clearly distinguished by PCR-RFLP analysis at six conserved single nucleotide polymorphisms in common wheat and wild and cultivated einkorn, emmer and timopheevi wheat. The Q locus genotype of Triticum sinskajae, which is one of the einkorn wheat species and exhibits free-threshing trait, was determined to be qq as expected. This simple PCR-RLFP-based genotyping method should serve as a useful tool in studying the origin of Q and thus wheat evolution after domestication and the following widespread cultivation.

• 日本遺伝学会の論文
• 2009-06-25

著者

• Mori N

  Lab. Of Plant Genetics Graduate School Of Agricultural Sci. Kobe Univ.

• Mori Naoki

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• Mori N

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• MORI Naoki

  Laboratory of Genetics, Faculty of Agriculture, Kobe University

• NAKAMURA Chiharu

  Laboratory of Plant Genetics, Faculty of Agriculture, Kobe University

• ASAKURA Nobuaki

  Laboratory of Biology, Faculty of Engineering, Kanagawa University

• OHTSUKA Ichiro

  Laboratory of Biology, Faculty of Engineering, Kanagawa University

• Mori Naoki

  神戸大学農学部

• Nakamura C

  Lab. Of Plant Genetics Dep. Of Agroenvironmental Sci. Graduate School Of Agricultural Sci. Kobe Univ

• Mori N

  Laboratory Of Plant Genetics Department Of Biological And Environmental Science Graduate School Of A

• Ohtsuka Ichiro

  Laboratory Of Biology Faculty Of Engineering Kanagawa University

• Nakamura Chiharu

  Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu

• Mori Naoki

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Nakamura Chiharu

  Laboratory Of Plant Genetics Department Of Agroenvironmental Science Graduate School Of Agricultural

• Mori Naoki

  Laboratory Of Chemical Ecology Graduate School Of Agriculture Kyoto University

• Asakura Nobuaki

  Laboratory Of Biology Faculty Of Engineering Kanagawa University

• Mori Naoki

  Laboratory Of Plant Genetics Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University

• NAKAMURA Chiharu

  Laboratory of Genetics, Faculty of Agriculture, Kobe University

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