Differential regulation of transcript accumulation and alternative splicing of a DREB2 homolog under abiotic stress conditions in common wheat
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概要
- 論文の詳細を見る
A number of cold responsive (Cor)/late embryogenesis abundant (Lea) genes are induced by both low temperature (LT) and dehydration. To understand the molecular basis of cold acclimation and its relationship with drought stress response in wheat seedlings, we isolated a DREB2 homolog Wdreb2, which is the candidate gene for a transcription factor of the Cor/Lea genes. The Wdreb2 expression was activated by cold, drought, salt and exogenous ABA treatment. Detailed expression studies of Wdreb2 indicated the involvement of two distinct pathways of its activation, a drought and salt stress-responsive pathway and a cold-responsive pathway. The transient expression analysis showed that the Wrab19 expression was directly activated by the WDREB2 transcription factor in wheat cells. Three transcript forms of Wdreb2 (Wdreb2α, Wdreb2β and Wdreb2γ) were produced through alternative splicing. Under drought and salt stress conditions, the amount of the Wdreb2β form remained fairly constant during 24-hour treatment, while those of the Wdreb2α and Wdreb2γ forms showed transient increases. On the other hand, the LT treatment resulted in increased transcript levels of all three forms of Wdreb2. Thus, under the LT and drought/salt stress conditions the amount of the WDREB2 transcription factors in wheat is differentially controlled by the level of transcription and alternative splicing.
- 日本遺伝学会の論文
- 2006-04-25
著者
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NAKAMURA Chiharu
Laboratory of Plant Genetics, Faculty of Agriculture, Kobe University
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Nakamura C
Lab. Of Plant Genetics Dep. Of Agroenvironmental Sci. Graduate School Of Agricultural Sci. Kobe Univ
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Nakamura Toshiki
Tohoku National Agriculture Research Center
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Nakamura Toshiki
Tohoku National Agricultural Experiment Station
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Kobayashi Fuminori
Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu
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Takumi Shigeo
Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu
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Nakamura Toshiki
Tohoku National Agriculture Res. Center National Agriculture And Food Res. Organization
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Takumi S
Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu
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Takumi Shigeo
Dep. Of Biological Sciences Stanford Univ.
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Nakamura Chiharu
Lab. Of Plant Genetics Dep. Of Agroenvironmental Sci. Graduate School Of Agricultural Sci. Kobe Univ
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Nakamura C
Kobe Univ. Kobe Jpn
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EGAWA Chikako
Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agricul
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ISHIBASHI Machiko
Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agricul
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Egawa Chikako
Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu
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Nakamura Chiharu
Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu
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Ishibashi Machiko
Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu
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Nakamura Chiharu
Laboratory Of Plant Genetics Department Of Agroenvironmental Science Graduate School Of Agricultural
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NAKAMURA Chiharu
Laboratory of Genetics, Faculty of Agriculture, Kobe University
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