Mitochondrial biogenesis as revealed by mitochondrial transcript profiles during germination and early seedling growth in wheat

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概要

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• Germination of imbibed embryos is the initial stage of plant development that is accompanied by the burst of mitochondrial respiration. To understand the process of mitochondrial biogenesis during this critical stage in wheat development, we monitored changes in mitochondrial transcript profiles during the first 3 days by adopting a newly devised macroarray system. The whole experiment was conducted in the dark to avoid influences of photosynthesis. Dry quiescent embryos started respiration rapidly after imbibition and the rate of oxygen uptake increased to peak at the first day followed by a continuous decrease until the third day under this condition. Both the cytochrome and alternative electron transport pathways appeared to contribute to this initial burst. Shoot and root growth was also remarkable during this period. Mitochondrial transcriptome was studied by macroarray analysis using 28 mitochondrial protein-coding genes, 4 nuclear encoded mitochondria-targeted genes and 2 nuclear genes as control. All transcripts were present in dry embryos at different initial levels, and a large variability was observed in their abundance among individual genes throughout the tested period. Gene expression was categorized into four clusters according to the profiles of individual transcript accumulation. A majority of the genes encoding subunits of the respiratory complexes belonged to two major clusters, the time course of transcript accumulation of one cluster agreeing with that of respiratory development and the other remaining at high constant levels. The macroarray system devised in this study should be useful in monitoring mitochondrial biogenesis under various growth conditions and at different developmental stages in cereals.

• 日本遺伝学会の論文
• 2007-10-25

著者

• Kadowaki Koh-ichi

  Genetic Diversity Department National Institute Of Agrobiological Sciences

• Khanam Sakina

  Laboratory Of Plant Genetics Department Of Agroenvironmental Science Graduate School Of Agricultural

• Kadowaki Koh-ichi

  Genetic Diversity Dep. National Inst. Of Agrobiological Sciences

• Nakamura Chiharu

  Lab. Of Plant Genetics Dep. Of Agroenvironmental Sci. Graduate School Of Agricultural Sci. Kobe Univ

• Naydenov Nayden

  Lab. Of Plant Genetics Dep. Of Agroenvironmental Sci. Graduate School Of Agricultural Sci. Kobe Univ

• Naydenov Nayden

  Laboratory Of Plant Genetics Faculty Of Agriculture And Graduate School Of Science And Technology Ko

• Nakamura Chiharu

  Laboratory Of Plant Genetics Department Of Biological And Environmental Science Faculty Of Agricultu

• Nakamura Chiharu

  Laboratory Of Plant Genetics Department Of Agroenvironmental Science Graduate School Of Agricultural

• Naydenov Nayden

  Laboratory Of Plant Genetics Department Of Agroenvironmental Science Graduate School Of Agricultural Science Kobe University

• NAKAMURA Chiharu

  Laboratory of Genetics, Faculty of Agriculture, Kobe University

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