Differential Expression of 3β-Hydroxysteroid Dehydrogenase mRNA in Rat Testes Exposed to Endocrine Disruptors
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概要
- 論文の詳細を見る
Expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) is mainly found in the Leydig cells from which steroid hormones are biosynthesized in the testes. To investigate whether endocrine disruptors affect the microenvironment of the testes, the mRNA expression of 3β-HSD as a molecular marker for androgen biosynthesis was analyzed in rat testes exposed to several endocrine disruptors using a reverse transcription-polymerase chain reaction technique. Testosterone [50, 200, and 1,000 μg/kg body weight (BW)], flutamide (1, 5, and 25 mg/kg BW), ketoconazole (0.2, 1, 5, and 25 mg/kg BW), diethylhexyl phthalate (10, 50, and 250 mg/kg BW), nonylphenol (10, 50, 100, and 250 mg/kg BW), octylphenol (10, 50, and 250 mg/kg BW), and diethylstilbestrol (10, 20, and 40 μg/kg BW) were orally administered to 4-week-old Sprague-Dawley male rats for 3 weeks. Although testosterone at a low dose (50 μg/kg/day) increased the expression of 3β-HSD mRNA, it was significantly decreased in the rats treated with 200 or 1,000 μg/kg/day testosterone compared with the control group (p<0.05). Furthermore, ketoconazole, diethylhexyl phthalate, nonylphenol, octylphenol, and diethylstilbestrol caused significant downregulation of 3β-HSD mRNA in the testes at all doses (p<0.05). However, flutamide remarkably increased the level of 3β-HSD mRNA in the testes (p<0.05). These results suggest that endocrine disruptors may influence androgen biosynthesis in the testes by alteration of 3 β-HSD mRNA expression.
- 日本繁殖生物学会の論文
著者
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Nam Sang-yoon
College Of Veterinary Medicine Chungbuk National University
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Lee Beom
College Of Veterinary Medicine Chungbuk National University
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YUN Young
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National Univ
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Lee Beom
College Of Veterinary Medicine And Research Institute Of Veterinary Medicine Chungbuk National Unive
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LEE Se-Ra
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National Univ
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YON Jung-Min
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National Univ
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BAEK In-Jeoung
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National Univ
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NAHM Sang-Soep
College of Veterinary Medicine, Konkuk University
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Lee Se-ra
College Of Veterinary Medicine And Research Institute Of Veterinary Medicine Chungbuk National Unive
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Yon J‐m
College Of Veterinary Medicine And Research Institute Of Veterinary Medicine
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Kwak Dong
College Of Veterinary Medicine And Research Institute Of Veterinary Medicine Chungbuk National Unive
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Kwak Dong-hoon
College Of Natural Sciences And Research Institute For Basic Sciences Of Wonkwang University
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Yun Young
College Of Natural Sciences Department Of Biological Sciences Pusan National University
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Nam Sang-yoon
College Of Veterinary Medicine And Research Institute Of Veterinary Medicine
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Lee Jeung-eun
College Of Veterinary Medicine And Research Institute Of Veterinary Medicine Chungbuk National Unive
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Baek In-jeoung
College Of Veterinary Medicine And Research Institute Of Veterinary Medicine
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KIM Hwan-Hee
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National Univ
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JEONG Jae-Hwang
Department of Bio-industry, Seowon University
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Yon Jung-Min
College of Veterinary Medicine and Research Institute of Veterinary Medicine
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NAM Sang-Yoon
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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BAEK In-Jeoung
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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KIM Hwan-Hee
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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YON Jung-Min
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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NAHM Sang-Soep
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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KWAK Dong-Hoon
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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LEE Jeung-Eun
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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