Enhanced Expression of Transferrin Receptor Confers UV-resistance in Human and Monkey Cells

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概要

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• One of the most intriguing biological subjects is cell-surface molecules that regulate the susceptibility of human cells to cell-killing effects after irradiation with far-ultraviolet light (UV, principally 254nm wavelength). Human RSa cells have unusual sensitivity to UV-induced cell-killing. We searched for molecules on the cell-surface of RSa cells that were present in different amounts as compared to a variant of these cells, UV^r-1 cells, which have increased resistance to UV cell-killing. Among the 21 molecules examined, the amount of transferrin receptor (TfR) protein was found to be 2-fold higher in UV^r-1 cells compared with in RSa cells. The amounts of this protein were also higher in the UV-resistant hematopoietic cell lines, CEM6 and Daudi, as compared to the UV-sensitive cell lines, Molt4 and 697. Culturing of UV^r-1 cells in a medium containing anti-transferrin antibodies resulted in sensitization of the cells to UV cell-killing as demonstrated by colony formation assay. Similar results were observed by treatment of the cells with TfR siRNA. In contrast, overexpression of TfR protein led to a resistance to UV cell-killing in RSa cells and monkey COS7 cells as demonstrated by both colony formation and apoptosis assay. In TfR-overexpressing cells, reduction of p53 and Bax protein was observed after UV-irradiation. Thus, TfR expression appears to be involved in the regulation of UV-resistance, possibly via modulation of the amount of p53 and Bax protein.

• 日本放射線影響学会の論文
• 2005-12-28

著者

• Chen Zheng

  Department Of Environmental Biochemistry Graduate School Of Medicine Chiba University

• Chen Zheng

  千葉大学 医学研究院環境影響生化学

• Suzuki Nobuo

  Dep. Of Environmental Biochemistry Graduate School Of Medicine Chiba Univ.

• Suzuki Nobuo

  千葉大学 教育

• Suzuki Toshikazu

  Department of Internal Medicine, Daido Hospital

• SUZUKI Nobuo

  Department of Surgery, Tsuruoka City General Hospital

• NOMURA Jun

  Faculty of Education, Chiba Univ.

• NOMURA Jun

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

• Suzuki Toshikazu

  Department Of Environmental Biochemistry Graduate School Of Medicine Chiba University

• Suzuki N

  Dep. Of Environmental Biochemistry Graduate School Of Medicine Chiba Univ.

• Nomura Jun

  Department Of Environmental Biochemistry Graduate School Of Medicine Chiba University

• Suzuki Toshikazu

  Dep. Of Environmental Biochemistry Graduate School Of Medicine Chiba Univ.

• Suzuki Toshikazu

  Graduate School Of Medicine Chiba University

• Suzuki Toshikazu

  Department Of Biuchemistry And Cell Biology.nalional Institute Of Infectious Diseaser

• Sato Nobutoshi

  Kanazawa Univ. Ishikawa Jpn

• Suzuki Nobuo

  Department Of Applied Physics Tohoku University

• Suzuki Nobuo

  Department Of Applied Physics Faculty Of Engineering Tohoku University

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