Cold-Active Polygalacturonase from Psychrophilic-Basidiomycetous Yeast Cystofilobasidium capitatum Strain PPY-1

スポンサーリンク

概要

• 論文の詳細を見る

• We purified and characterized a cold-active polygalacturonase (PG) from the extracellular fraction of Cystofilobasidium capitatum strain PPY-1. The purified PG from strain PPY-1 has a molecular mass of about 44 kDa, and exhibited high activity at 0 °C, although its optimum temperature was 45 °C. Although the Km value for polygalacturonate as a substrate at 45 °C was found to be 11.2 mg/ml, it decreased gradually with decreasing temperature, and it was 0.66 mg/ml at 0 °C. Moreover, its cleavage pattern was of the endo-type. These findings might indicate that PG from strain PPY-1 is a novel type of cold-active endo-PG that is able to degrade pectin compounds at low temperatures.

• 社団法人 日本農芸化学会の論文
• 2005-02-23

著者

• NAKAGAWA Tomoyuki

  Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University

• MIYAJI TATSURO

  Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture

• TOMIZUKA NOBORU

  Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture

• Tomizuka Noboru

  National Institute of Bioscience and Human-Technology (NIBH)

• Nakagawa Tomoyuki

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Miyaji Tatsuro

  Department Of Bioproduction Science School Of Horticulture Chiba University:(present Address) Depart

• NAGAOKA Toshinori

  Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture

• Nagaoka Toshinori

  Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture

• Nakagawa Tomoyuki

  Department Of Chemistry And Biochemistry Graduate School Of Engineering Kyushu University

関連論文

• Molecular Characterization of Two Genes with High Similarity to the Dihydroxyacetone Synthase Gene in the Methylotrophic Yeast Pichia methanolica
• Chemical Properties of Sperm Whale Myoglobins Reconstituted with Monopropionate Hemins
• Isozymes of Methanol Oxidase in a Methanol-Utilizing Yeast, Pichia methanolica IAM 12901
• Effect of Oxygen on the Growth of Clostridium butyricum(Type Species of the Genus Clostridium), and the Distribution of Enzymes for Oxygen and for Active Oxygen Species in Clostridia
• Analysis of Alcohol Oxidase Isozymes in Gene-Disrupted Strains of Methylotrophic Yeast Pichia methanolica
• Purification and Properties of Gentisate 1,2-Dioxygenase from Rhodococcus erythropolis S-1
• Purification and Properties of 3 Types of Monohydroxybenzoate Oxygenase from Rhodococcus erythropolis S-1
• Purification and Properties of Phthalate Oxygenase from Rhodococcus erythropolis S-1
• A New Peptidase Specific to D-Amino Acid Peptides from a Nocardia sp.
• Purification and Properties of a Peptidase from Nocardia orientalis Specific to D-Amino Acid Peptides(Biological Chemistry)
• Isolation and Partial Characterization of Homogeneous Nitrite Reductase from a Cyanobacterium, Aphanothece sacrum
• The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica
• Cold-Active Pectinolytic Activity of Psychrophilic-Basidiomycetous Yeast Cystofilobasidium capitatum Strain PPY-1
• cDNA Cloning And Sequence Analysis Of A Precursor For Endothelin-3 From Mouse Intestine
• Inhibitory Effect of Alginic Acids on Hyaluronidase and on Histamine Release from Mast Cells
• Assessment of the Microbial Contribution to the Processing of Salted Salmon Roe (Sujiko)
• Cold-Active Polygalacturonase from Psychrophilic-Basidiomycetous Yeast Cystofilobasidium capitatum Strain PPY-1
• Degradation of Dimethyl Disulfide by Pseudomonas fluorescens Strain 76
• The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica
• Molecular Characterization of Two Genes with High Similarity to the Dihydroxyacetone Synthase Gene in the Methylotrophic Yeast Pichia methanolica
• Degradation of Dimethyl Disulfide by Pseudomonas fluorescens Strain 76
• Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Identification of Soluble Proteins and Interaction with Mannan in Mucilage of Dioscorea opposita Thunb. (Chinese Yam Tuber)

もっと見る 閉じる

スポンサーリンク