Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily
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概要
- 論文の詳細を見る
Human TLR2 subfamily recognizes bacterial lipoproteins (BLP) and peptidoglycan (PGN). According to the genome information, chicken has structural orthologs of TLRs 1 and 2, in addition to TLRs3, 4, 5 and 7. Chicken has two additional TLRs, TLR15 and TLR21, which orthologs human lacks. The chicken (ch)TLR1 and 2 genes are individually duplicated to encode for two different proteins, chTLRs, chTLR1-1, 1-2, 2-1 and 2-2, of the TLR2 subfamily. Here we investigated the functional profile of these TLR2 subfamily proteins of chicken. By NF-κB reporter assay using HEK293 cells, we found that chTLR2-1 and chTLR1-2 cooperatively signal the presence of PGN. A combination of chTLR2-1 and chTLR1-2 also most efficiently recognized diacylated BLP, Malp-2, while the combination of chTLR2-1 and chTLR1-1 failed to recognize Malp-2. All combinations, however, recognized triacylated BLP, Pam3. Consistent with these results, human TLR2-stimulating mycobacteria preparations, BCG-cell wall and cell lysate of Mycobacterium avium, induced activation of NF-κB in cells expressing chTLR2-1 and 1-2 and to lesser extents, cells with chTLR2-2 and either of chTLR1. Strikingly, expression of either of these alone did not activate the reporter for NF-κB. These chTLRs are likely to have the combination functional feature as in the human TLR2 subfamily. Confocal and immunoprecipitation analyses of human cell transfectants showed that they cluster on the cell surface by a physical molecular association, causing all of them to merge and coprecipitate. These results suggest that chTLR2 subfamily members discriminate between their ligands by combinational events.
- Pergamonの論文
著者
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Matsumoto Misako
北海道大学 医学研究科免疫学
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Seya Tsukasa
大阪府立成人病センター研究所 免疫学部門
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Seya Tsukasa
Department Of Immunology Osaka Medical Center For Cancer And Cardiovascular Diseases:probrain
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松本 美佐子
Department Of Microbiology And Immunology Hokkaido University Graduate School Of Medicine
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Funami Kenji
Department Of Microbiology And Immunology Hokkaido University Graduate School Of Medicine
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Saeki Y
Department Of Biochemistry The University Of Texas Health Center
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Shida Kyoko
Osaka Medical Center For Cancer And Cardiovascular Diseases Osaka
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