Xylose induces the phyllosphere yeast Pseudozyma antarctica to produce a cutinase-like enzyme which efficiently degrades biodegradable plastics(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
There is a need to speed up the degradation of used agricultural mulch films that are made of biodegradable plastics (BPs) in the field. Treating them with BP-degrading enzymes could be a solution to this problem. A cutinase-like enzyme of yeast Pseudozyma antarctica (PaE) has wide specificity of BPs degradation, but needs to be produced efficiently. Here we report that the production of PaE by P. antarctica can be increased by using xylose as carbon source. BP-degradation activity was analyzed using a polybutylene succinate-co-adipate (PBSA) emulsion as the substrate. Strain P. antarctica GB-4(1)W was found to be the best PaE producer among the tested strains. Using a 5-L jar fermentor with xylose fed-batch cultivation, high PaE productivity could be maintained and about 21 U/ml of PaE was obtained in 120 h. This amount was 100 times higher than the amount that we obtained previously (0.21 U/ml by flask cultivation using glycerol as carbon source). Under repeated xylose fed-batch cultivation with 24 h intervals, the maximum PaE production rate (0.34 U/ml/h) was maintained and the highest PaE productivity (28,000 U/2 L/d) was repeatedly obtained for 7 intervals. The activity of filtered jar-culture (crude PaE) was stable over 12 weeks at 4℃. Commercially available BP mulch films (20 μm thickness, cut into 1-cm-squares) were completely degraded by submerging them in crude PaE (2 U/ml) at 30℃ in 24 h. These results indicated that concentrated PaE can rapidly degrade the strength of BP mulch films in the field so that they do not interfere with plowing.
- 公益社団法人日本生物工学会の論文
著者
-
YOSHIDA Shigenobu
National Institute for Agro-Environmental Sciences
-
Koitabashi Motoo
National Agricultural Research Center For Kyushu Okinawa Region
-
Kitamoto Hiroko
National Institute For Agro-environmental Sciences
-
Fujii Takeshi
National Inst. For Agro-environmental Sciences
-
Shinozaki Yukiko
National Institute for Agro-environmental Sciences
-
Watanabe Takashi
National Institute for Agro-environmental Sciences
-
Sameshima-Yamashita Yuka
National Institute for Agro-environmental Sciences (NIAES)
-
Fukuoka Tokuma
National Institute of Advanced Industrial Science and Technology (AIST)
-
Shinozaki Yukiko
National Institute for Agro-environmental Sciences (NIAES):Japan Society for the Promotion of Science
-
Kitamoto Hiroko
National Institute for Agro-environmental Sciences (NIAES)
関連論文
- An Enzyme-Linked Immunosorbent Assay (ELISA) to Determine the Specificity of the Sugar-Binding Protein NgcE, a Component of the ABC Transporter for N-Acetylglucosamine in Streptomyces olivaceoviridis
- 1Fp23 植物から分離された糸状菌による生分解性プラスチックの分解と分解酵素の活性(発酵生理学・発酵工学,一般講演)
- P-22 転換畑を水田にしたときのアンモニア酸化菌などの微生物叢の変化(ポスター発表)
- 06-107 水田転換畑土壌における硝化古細菌の群集構造(土壌生態系,研究発表)
- 6-23 古細菌の土壌における生態 : 転換畑土壌における古細菌および硝化古細菌の多様性(6.土壌生物,2008年度愛知大会)
- Aromatic Substances Inhibiting Wheat Powdery Mildew Produced by a Fungus Detected with a New Screening Method for Phylloplane Fungi
- 独立行政法人農業環境技術研究所 国際ワークショップ (2009年10月6日-7日) : 「農業研究におけるメタゲノミクスの展望」の開催報告
- 1A-7(P-32) 農耕地土壌における細菌、古細菌、真核生物のrDNAの定量の試み(口頭発表+ポスター発表)
- 11-212 土壌から直接法で長鎖DNAを抽出する方法の改良(方法論,研究発表)
- Effects of Full Annealing Heat Treatment on Long-term Creep Strength of 2.25Cr-lMo Steel Welded Joint
- Prediction of Creep Lifetime for Butt-Welded Joint of Type 304 Stainless Steel by Finite Element Method Incorporating Damage Variable
- 6-28 水田転換畑土壌における窒素動態と硝化菌の生態(6.土壌生物,2009年度京都大会)
- Creep Fracture Analysis of W Strengthened High Cr Steel Weldment
- Microstructural Investigations on Type IV Cracking in a High Cr Steel
- 土壌抽出RNAを用いた土壌微生物の遺伝子発現解析
- 05-087 クロロカテコール分解プラスミドがクロロ安息香酸分解菌の走化性に及ぼす影響(バイオレメディエーション,研究発表)
- 酵母(Candida antarctica)の非末端酸化系による炭化水素類からの糖脂質型バイオサーファクタント(マンノシルエリスリトールリピド)の生成
- Antifungal substances produced by fungal strain Kyu-W63 from wheat leaf and its taxonomic position
- 放線菌 Streptomyces coelicolor A3(2) における2つの(GlcNAc)_2輸送系の役割
- 放線菌 Streptomyces coelicolor A3(2) のキチナーゼ生産におけるDasRの機能解析
- Comparison of Enzymatic and Antifungal Properties between Family 18 and 19 Chitinases from S. coelicolor A3(2)
- High-multiplicity of Chitinase genes in Streptomyces coelicolor A3 (2)
- Shoot Blight and Leaf Spot of Blueberry Anthracnose Caused by Colletotrichum acutatum
- Two Alternatively Spliced Transcripts Generated from OsMUS81, a Rice Homolog of Yeast MUS81, Are Up-Regulated by DNA-Damaging Treatments
- SB-07-3(083) Effect of Microstructural Changes During Creep on The Creep Rate at 823K in Type 304 Heat Resistant Steel(Changes in Microstructure 2)
- Rhizopus Rot of Mulberry-grafted Saplings Caused by Rhizopus oryzae
- Biotin Induces Sporulation of Mulberry Anthracnose Fungus, Colletotrichum dematium
- 6-12 水田転換畑土壌におけるアンモニア酸化細菌の多様性(6.土壌生物,2010年度北海道大会)
- DNA-Binding Protein Involved in the Regulation of Chitinase Production in Streptomyces lividans
- Induction and Repression of a Streptomyces lividans Chitinase Gene Promoter in Response to Various Carbon Sources
- Chitinase System in Streptomyces
- Isolation of Effective 3-Chlorobenzoate-Degraders in Soil Using Community Analyses by PCR-DGGE
- Analysis of a Bacterial Community in 3-Chlorobenzoate-Contaminated Soil by PCR-DGGE Targeting the 16S rRNA Gene and Benzoate 1,2-Dioxygenase Gene (benA)
- Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
- Ethanol production from ensiled rice straw and whole-crop silage by the simultaneous enzymatic saccharification and fermentation process(ENVIRONMENTAL BIOTECHNOLOGY)
- 2,4-Dichrolophenoxyacetic Acid-degrading Genes from Bacteria Isolated from Soil in Japan : Spread of Burkholderia cepacia RASC-type Degrading Genes Harbored on Large Plasmids
- Cochliobolus heveicola sp. nov. (Bipolaris heveae) causes brown stripe of bermudagrass and Zoysia grass
- Nucleotide Sequence and Analysis of a Gene (chiA) for a Chitinase from Streptomyces lividans 66
- Nucleotide Sequence and Expression of a Gene (chiB) for a Chitinase from Streptomyces lividans
- Evaluation of Methods of Determining Humic Acids in Nucleic Acid Samples for Molecular Biological Analysis
- 放線菌 Streptomyces coelicolor A3(2) のDasDタンパク質は細胞質局在型β-N-アセチルグルコサミニダーゼとして機能する
- 3F1012 P64 β-ケトアジピン酸走性に関与するBurkholderia sp. NK8株の外膜ポリン遺伝子(3F 神経・感覚、神経回路・脳の情報処理、行動,日本生物物理学会第49回年会)
- 農耕地土壌におけるメタゲノム解析研究の動向
- Blue mold of tomato caused by Penicillium oxalicum in Japan
- New cylindrocladium diseases of strawberry and coral bells caused by Cylindrocladium canadense
- 塩素化芳香族化合物分解菌のβ-ケトアジピン酸走化性に関与する外膜タンパク遺伝子(2011年度大会一般講演要旨)
- 6-4 放線菌Streptomyces coelicolorのdasD遺伝子のキチン分解における機能の解明(6.土壌生物)
- Extraction of Bacterial RNA from Soil : Challenges and Solutions
- ドットブロットハイブリダイゼーション法を用いた土壌中rDNAの判別定量(2009年度大会一般講演要旨)
- Assessment of the Effects of Genetically Modified Pseudomonas spp. Expressing Chitinase on the Soil Microbial Community in the Cucumber Rhizosphere
- Biological Control of Rhizoctonia damping-off of Cucumber by a Transformed Pseudomonas putida Strain Expressing a Chitinase from a Marine Bacterium
- 農耕地土壌における細菌,真核生物および古細菌のrDNA存在比の推定 : 古細菌rDNA存在比に及ぼす土壌環境要因の解析
- Labor-saving Preservation of Powdery Mildew of Strawberry by Sterilized Seedling Culture
- Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films(METHODS)
- 3PT184 3-クロロ安息香酸による3-クロロ安息香酸分解細菌Burkholderia sp.NK8外膜ポリン遺伝子の転写促進(日本生物物理学会第50回年会(2012年度))
- Xylose induces the phyllosphere yeast Pseudozyma antarctica to produce a cutinase-like enzyme which efficiently degrades biodegradable plastics(ENVIRONMENTAL BIOTECHNOLOGY)
- Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films