J024034 放電駆動衝撃波装置を用いた衝撃波照射が与える細胞応答に関する検討([J02403]バイオにおける流れと熱・物質移動(3))

概要

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The characteristics of shock wave have a great potential for medical applications. Extracorporeal Shock Wave Lithotripsy is one such medical application. Recently, it is suggested that angiogenesis, Drug Delivery System and Gene Transfer using low-level shock wave. We developed small shock wave device for minimally invasive surgery. In this device, shock waves are generated by electrical discharge. We evaluated this device with visualization of shock wave and measurement of pressure. The results of visualization showed that shock wave converged toward the second focal point after 15 microsecond of electrical discharge, and this device could produce an average pressure of 2.32 MPa with standard deviation of 0.89 MPa. Then we had two experiments to investigate the effects of shock wave on biological cells by our device. In first experiment, cells exposed to shock wave were stained by Calcein-AM and PI. Cells were stained red in the small region; the size was about 1.5mm. This result showed that our device could provide effects locally. In another experiment, we investigate the variations of calcium ion concentration during shock wave irradiation. We observed calcium-ion elevation depending on shock wave irradiation.
一般社団法人日本機械学会の論文
2011-09-11