コガネバナ茎力ルスの生長とフラボノイド生成に及ぼす培養期間の影響

概要

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Time course changes in the callus growth and flavonoid content in St-20 line derived from Scutellaria baicalensis GEORGI stem callus cultures were determined on Linsmaier-Skoog's culture medium containing 10^<-6> M IAA and 10^<-5> M kinetin. When 3% sucrose was added to the medium, a logarithmic callus growth phase was observed after the lapse of 20-40 day-cultivation, and stationary and decelation growth phases after 50-60 day-cultivation. Flavonoid content increased gradually during the stationary and decelation growth phases and the content of the glucosides, baicalin and wogonin 7-O-glucuronide reached the highest values after 60 day-cultivation. Among the carbon sources tested, 5% maltose accerelated the rate of growth of St-20 line most effectively. On the 5% maltose-containing medium, a logarithmic growth phase was observed after the lapse of 10 days and a stationary growth phase after 50 day-cultivation. The flavonoid content of St-20 cells, stationary cultured for 70 days in the dark, was found to be increased (baicalin, 16 mg/10 ml medium) by the addition of 5% maltose to the medium. Brief light irradiation of the cells which had been cultured for 20 days in the dark effectively increased the rate of callus growth and the flavonoid content of the callus.
日本生薬学会の論文
1986-03-20