コガネバナ培養組織の2段階培養および再分化根の培養におけるフラボノイド生成

概要

論文の詳細を見る
A two-stage liquid culture system of Scutellaria baicalensis callus cultures (St-20 line), useful for the purpose of promoting its flavonoid production was established. Callus cultures were first grown in LS medium for 20 days and subsequently in White's medium containing 10^<-7> M IAA and 5% maltose for 20 days. Consequently, the baicalin and wogonin 7-O-glucuronide contents increased to 9.07% and 6.33% of dry weight, respectively, as compared with 4.0% and 1.5%, respectively, in the ordinary batch cultures. These results showed that the two-stage cultures yielded as much flavonoids as the root (Wogon) of the intact plant did. The flavonoid content of the callus obtained by the forty day two-stage liquid culture method was higher than that of the roots differentiated from callus cultures.
日本生薬学会の論文
1989-06-20