高速液体クロマトグラフィーによる食品中の合成甘味料およびそれら分解物の同時分析

概要

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A simple and rapid method has been developed for determining 4 sweeteners of acesulfame potassium (AK), sodium saccharin (SA), aspartame (APM), neotame (NE) and 2 degradation products of diketopiperazine (DKP) and dimethylbutyl-aspartyl phenylalanin (DMB-AP) in various foods by high performance liquid chromatography (HPLC). These compounds in foods were extracted in ultrasonic bath, and the extract was loaded onto a strata-X-C, cation-exchange and reverse-phase cartridge. The HPLC separation was performed on a Inertsil ODS-4 column (5 μm, 4.6 mm i.d. × 150 mm) using 0.01 mol/L phosphate buffer (pH3.5) and methanol as a mobile phase. The injection volume, flow rate and column temperature were 10 μL, 1.0 mL/min and 40℃, respectively. A UV absorbance detector was set to 210 nm. The recoveries of 4 sweeteners and 2 degradation products from foods added at the level 0.20 g/kg and 0.04 g/kg in HPLC were 82.3-104.9% and 80.4-108.1%, respectively. The determination limit of these compounds was 0.005 g/kg. Nine commercial foods indicated the using of NE were determined by the proposed method. NE was detected in 6 foods in the range of 0.02-0.09 g/kg, AK and APM were detected in 2 foods, simultaneously.
2012-04-23