カリン果実中の青酸配糖体およびその分解物の分布と消長

概要

論文の詳細を見る
A method has been developed for determining amygdalin (AM) and its degradation products, benzyl alcohol (BeOH), benzaldehyde (BAL) and benzoic acid (BA) in Chinese quince fruit utilizing high performance liquid chromatography (HPLC). A sample was extracted with 0.05 mol/L citric acid solution (pH2.2) and cleaned in an InertSep C18 cartridge and an InertSep NH_2 cartridge. The HPLC separation was performed on an Inertsil ODS-4 column using acetonitrile-0.01 mol/L phosphate buffer (pH2.0) (15:85) in the mobile phase. A UV monitor was used for detection. The effects during growth period on the amount of 4 components of sarcocarp and seed of Chinese quince was examined. AM content in seed increased proportionally each month. This increase was especially significant in seed retrieved in November. AM was detected in commercially available Chinese quince in the range of 0.03-0.06 mg/g sarcocarp, 0.21-1.75 mg/g seed. BA was not detected in any samples.
2014-04-25