87. イネの根特異的ストレス応答RSOs-PR10遺伝子のプロモーター解析(口頭発表,植物化学調節学会第46回大会)

概要

論文の詳細を見る
We have identified RSOsPR10 (Root Specific Oryza sativa Pathogenesis-Related 10) as the root specific protein induced by drought and salt treatments in rice. Expression of RSOsPR10 was up-regulated by JA and ACC, while SA suppressed its induction at the transcription level (Takeuchi et al., PCP, 2011). Here, we constructed transgenic rice plants expressing GUS under the control of 1.9- or 4-kb RSOsPR10 promoter region. The transgenic seedlings (T1) were untreated or treated with JA, and measured the expression levels of GUS mRNA in roots and shoots by qRT-PCR. On the transgenic rice plants with 1.9 kb::GUS, the GUS expression was relatively low levels in roots and shoots, and not increased by JA treatment. On the other hand, the higher GUS expression in the root of the transgenic rice plants with 4 kb::GUS was detected comparing to those in shoots, and the expression was significantly up-regulated by JA treatment only in the root. Next, we investigated the expression pattern of GUS staining in the root of 4 kb::GUS transgenic plants treated with or without JA. As a result, the GUS staining was observed at the roots, and the intense signals were detected after JA treatment at the early developed lateral roots. These results indicated that some cis-elements regulating root specific or stress responsive RSOsPR10 expression are in 2-4 kb RSOsPR10 upstream region.
植物化学調節学会の論文
2011-10-03