10.トウモロコシ幼葉鞘先端におけるIAA合成と細胞・組織構造との関係性の検討(口頭発表)

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Auxin is an important phytohormone and acts in many physiological events in plants. Especially, it has long been studied about its role in tropic curvature in glass coleoptiles. However, the production/biosynthesis of IAA at the coleoptile tip region was not approved in general. Our previous studies showed that the maize coleoptile 2-mm tip sections have an ability of de novo IAA biosynthesis at least until 2hrs after the cutting. However, when the tissues were chopped or homogenized, the increase of IAA amount was almost suppressed. To maintain the activity in the chopped samples, we had checked about the effects of buffer conditions, enzyme stabilizers, co-factors, metals, metal chelating agents, and other chemicals. However, we could not find any conditions to keep the IAA synthetic activity. Therefore, it was postulated that some specific cell/tissue structures are required for the IAA biosynthesis in the tip region. In the present work, we investigated whether the tip structure is important for the IAA biosynthetic activity. When the 2-mm tips were chopped into 1-mm or 0.5mm blocks, the activity was decreased about 40% or 70%, respectively. One or two cutting in longitudinal direction caused about 30% reduction, but in horizontal cutting would not show such large decrease. We are now investigating the relationship between IAA biosynthetic activity and cell/tissue structure in maize coleoptile tip regions.
植物化学調節学会の論文
2009-10-06

10.トウモロコシ幼葉鞘先端におけるIAA合成と細胞・組織構造との関係性の検討(口頭発表)

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