10.イネ幼葉鞘を用いたマイクロアレイによるIAA生合成遺伝子の探索(口頭発表)

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While Arabidopsis YUCCA, AAO, NIT, CYP72B2 and CYP79B3 genes have been reported as IAA synthesis genes, there is no direct evidence that they actually work in plants. In monocot coleoptiles, it has been shown for a long time that IAA is produced in very tip region and transported in a polar manner, and our previous works revealed that the IAA is synthesized de novo from tryptophan in the tip region of maize coleoptiles. We here tried to identify the IAA biosynthesis genes through Microarray analysis using rice coleoptiles. Apical (0-1.5 mm) and subapical (3-4.5 mm) parts of coleoptiles were sectioned from 4-day-old rice seedlings and mRNA was extracted from them. Microarray was performed according to Rice Genome Resource Center's protocol for Rice Oligo Microarray Kit, 22K from Agilent. We found 133 genes strongly expressing in the apical region. RT-PCR on 20 genes of them revealed that at least 15 genes expressed specifically in the apical region. Also, among six YUCCA-like genes, five AO-like genes, two NIT-like genes and forty-two P450-like genes found in the 22K rice Microarray targets, only three P450-like genes belonging to CYP71, not CYP79, family had the apical specific expression. Since 22K rice Array Slide does not cover all genes of rice (37554 genes), we are planning to perform 44K rice Microarray analysis using coleoptiles treated with IAA transport inhibitor NPA, adding to apical and subapical region of coleoptile.
植物化学調節学会の論文
2007-10-05

10.イネ幼葉鞘を用いたマイクロアレイによるIAA生合成遺伝子の探索(口頭発表)

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