2A2-A02 オンチップ細胞計測に関する研究 : その2:細胞内計測のためのナノツールの細胞導入と細胞内レーザ操作

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We developed injection of nanotool for intracellular measurement in a microfluidic chip. We have developed on-chip cell measurement of using functional gel-tool. We classified the cell measurement method in four categories. In this paper, we proposed functional nanotool included in fusogenic liposome for cell injection and intracellular measurement. The nanotool was modified by fluorescent dye for environment measurement. The nanotool was included in fusogenic liposome impregnated with photo chromic chemical. The liposome including the nanotool was manipulated by optical tweezers and contacted to the cell. After contact of the liposome to the cell, the liposome was adhered to the cell membrane by membrane fusion induced by UV-ray. Injected nanotool was manipulated by optical tweezers. Intracellular temperature was measured by detecting the fluorescence intensity of the nanotool. We demonstrated photo-induced membrane fusion of the liposome and manipulation of injected nanotool in the chip.
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