2A2-A01 オンチップ細胞計測に関する研究 : その1:マイクロ流体チップ内での単一ウイルス操作

概要

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We developed manipulation of single virus using optical tweezers and dielectrophoretic force in a microfluidic chip. We used a microfluidic chip made of poly (dimethylsiloxane) (PDMS). The chip has independent virus chamber and cell chamber to make quantitative analysis of the functions of influenza virus before and after infection to a cell. Dielectrophoretic force integrated inside virus chamber concentrates the virus. Concentrated viruses flow to the part for sample selection. The virus was transported to the cell chamber using optical tweezers. After virus transport, these chambers were isolated by photocrosslinkable resin. In this paper, we demonstrated concentration of influenza virus, manipulation of the single virus to transport the cell chamber, contact to a target cell in the chip and isolation of chambers in the microfluidic chip.
一般社団法人日本機械学会の論文