大腸菌Phageに於ける溶菌とPhage増加との関係について

概要

論文の詳細を見る
In these experiments we searched for relationships between growth of phage and cellular lysis of phage Tog and escherichia coli F_6 system, using 1-cysteine, penicillin G and hyaluronidase. The growth of phage was tested by one step growth experiments, and the cellular lysis by nephelometric examinations. Both methods were practiced in synthetic mediumcontaining the same concentration of drugs. Results were as follows. (1) In nephelometric examinations, 1-cysteine of 20γ/ml failed to inhibit the lysis of phage infected coli, but this lysis was very slow compared with control, and there, was no increase of active phage particles. (2) When penicillin G of 50 U/ml was added to the mixed culture of phage-hostcells and 1-cysteinne, the phage infected hostcells were lysed more rapidly than in above mentioned experiment (1), but there was no increasing of active phage particles. (3) When penicillin G of 50 U/ml and hyaluronidase of 200VRU/ml were added to the mixed culture of phage-hostcells and 1-cysteine, the phage infected hostcells were lysed still more rapidly than in the experiment (2), and active phage particles were found increasing.
千葉大学の論文
1953-11-28