磁性ビーズを用いた高速・自動バイオスクリーニングシステムの開発と抗がん薬剤の分子機構の解明(生体磁気・医療応用)

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Several cubic shaped single crystals of magnetite (40±6nm) were encapsulated in spherical polymer shells of 184±9nm diameter. They were used as magnetic carriers for affinity screenings of bioreceptor molecules (proteins) and their "affinity-partner" chemicals. The surfaces of the carrier particles were coated with poly-GMA, which suppressed non-specific chemical adsorption of molecules onto the carrier surfaces. This coating enabled high purity affinity screening. Rapid (for ca. 5min.) magnetic separation of the carrier magnetic beads, as well as simultaneous processing of 96 samples, enabled automated high throughput screening. The strong resistance of FG beads to organic solvents enables them to conjugate with drugs, which are mostly hydrophobic. Finally, we describe the molecular mechanism of anticancer drugs, MTX and several porphyrins based on our identification of their bioreceptors by our bioscreening system.
社団法人日本磁気学会の論文
2009-03-01