An Optimized Yeast Cell-Free System : Sufficient for Translation of Human Papillomavirus 58 L1 mRNA and Assembly of Virus-like Particles(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
We developed a yeast cell-free system suitable for in vitro translation of human papillomavirus 58 (HPV58) L1 mRNA. This system was systematically optimized resulting in enhanced translation efficiency. The optimal concentrations of potassium and magnesium ions observed were specific to the HPV58 L1 protein production. Supplementation with sucrose in the preparation of the yeast lysate greatly enhanced its stability. After optimization, protein production in this system was significantly superior to that produced by the rabbit reticulocyte (RRL) system. Finally, we demonstrated for the first time that virus-like particles (VLPs) were assembled from HPV58 L1 capsid protein in the yeast cell-free system. Thus, the system described here is a powerful tool for the HPV L1 protein production and will be useful for the study of VLP assembly and DNA encapsulation.
- 社団法人日本生物工学会の論文
- 2008-07-25
著者
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Wang Hong
Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee Universit
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Wang Xiao
Department of Medical Microbiology, Shandong University School of Medicine
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Liu Juan
Department of Medical Microbiology, Shandong University School of Medicine
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Zhao Wei
Department of Medical Microbiology, Shandong University School of Medicine
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Li Jing
Department Of Pharmacology Marine Drug And Food Institute Ocean University Of China
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Liu Juan
Department Of Medical Microbiology Shandong University School Of Medicine
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Zhao Wei
Department Of Medical Microbiology Shandong University School Of Medicine
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Liu Juan
Department Of Clinical Laboratories Peking Union Medical College Hospital Chinese Academy Of Medical
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Zhao Wei
Department Of Cardiovascular Medicine Shanghai Shuguang Hospital Affiliated With Shanghai University
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Zheng Yan
Department of Medical Microbiology, Shandong University School of Medicine
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Zhou Yabin
Department of Medical Microbiology, Shandong University School of Medicine
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Qi Mei
Department of Medical Microbiology, Shandong University School of Medicine
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Yu Han
Department of Medical Microbiology, Shandong University School of Medicine
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Tang Wei
Department of Medical Microbiology, Shandong University School of Medicine
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Yu Han
Department Of Medical Microbiology Shandong University School Of Medicine
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Wang Xiao
Department Of Medical Microbiology Shandong University School Of Medicine:university Of Queensland C
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Qi Mei
Department Of Medical Microbiology Shandong University School Of Medicine
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Tang Wei
Department Of Medical Microbiology Shandong University School Of Medicine
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Zheng Yan
Department Of Medical Microbiology Shandong University School Of Medicine:central Laboratory Jinan C
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Zheng Yan
Department Of Applied Chemistry School Of Chemical And Material Engineering Southern Yangtze Univers
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Wang Xiao
Department Of Electrical Engineering Faculty Of Engineering Osaka University
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Zhou Yabin
Department Of Medical Microbiology Shandong University School Of Medicine
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Li Jing
Department Of Agriculture Faculty Of Agriculture Kochi University
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Wang Hong
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Tang Wei
Department Of Anatomy Showa University School Of Medicine
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Wang Xiao
Department Of Applied Physics And Materials Research Center The Hong Kong Polytechnic University
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Yu Han
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Wang Xiao
Department Of Aeronautical Engineering Nagoya University
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Wang Hong
Department Of Medical Microbiology Shandong University School Of Medicine
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