Translational comparison of HPV58 long and short L1 mRNAs in yeast (Saccharomyces cerevisiae) cell-free system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Recently, we developed a yeast cell-free system for translation of human papillomavirus (HPV) 58 short L1 mRNA and VLP assembly in vitro. In the present study, we examined the translation efficiency of HPV58 long and short L1 mRNAs in our established system. Production of HPV58 long and short L1 proteins was significantly dependent on the amounts of the L1 mRNAs used and the time length of translation reaction. Compared with the long L1 mRNA, the short L1 mRNA consistently exhibited higher translation efficiency in all the experiments conducted. Supplementation of exogenous amino acids significantly enhanced translation of the long L1 mRNA in this system. Furthermore, a single amino acid leucine can be rate-limiting for translation of the long L1 mRNA in this system. Electron microscopy demonstrated that HPV58 virus-like particles (VLPs) were assembled from both long and short L1 capsid proteins in the yeast cell-free system.
著者
-
Wang Xiao
Department of Medical Microbiology, Shandong University School of Medicine
-
Liu Juan
Department of Medical Microbiology, Shandong University School of Medicine
-
Zhao Wei
Department of Medical Microbiology, Shandong University School of Medicine
-
Zhao Kong-Nan
University of Queensland Centre for Clinical Research, Royal Brisbane & Women's Hospital Campus
-
Zhao Kong-nan
University Of Queensland Centre For Clinical Research Royal Brisbane & Women's Hospital Cam
-
Liu Juan
Department Of Medical Microbiology Shandong University School Of Medicine
-
Zhao Wei
Department Of Medical Microbiology Shandong University School Of Medicine
-
Liu Juan
Department Of Clinical Laboratories Peking Union Medical College Hospital Chinese Academy Of Medical
-
Zhao Wei
Department Of Cardiovascular Medicine Shanghai Shuguang Hospital Affiliated With Shanghai University
-
Wang Xiao
Department Of Medical Microbiology Shandong University School Of Medicine:university Of Queensland C
-
Wang Xiao
Department Of Applied Physics And Materials Research Center The Hong Kong Polytechnic University
-
Wang Xiao
Department Of Aeronautical Engineering Nagoya University
関連論文
- Translational comparison of HPV58 long and short L1 mRNAs in yeast (Saccharomyces cerevisiae) cell-free system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Development of New Apparatus for Field Emission Measurement
- Mitogen-activated Protein Kinase Inhibitors Induce Apoptosis and Enhance the Diallyl Disulfide-induced Apoptotic Effect in Human CNE2 Cells
- Photoisomerization of Azobenzene Dendrimer Monolayer Investigated by Maxwell Displacement Current Technique : Atoms, Molecules, and Chemical Physics
- Comparison of Gene Transcription between Subcutaneous and Visceral Adipose Tissue in Chinese Adults
- An Optimized Yeast Cell-Free System : Sufficient for Translation of Human Papillomavirus 58 L1 mRNA and Assembly of Virus-like Particles(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Nutcracker phenomenon : A new diagnostic method of multislice computed tomography angiography
- VUV Photocatalytic Degradation of Toluene in the Gas Phase
- Analysis of Conformational Transition Effect on Maxwell Displacement Current : A Kinkable Rod Model
- Numerical Simulation of a Vertical, Turbulent Wall Fire in Space Vehicles in Weak Gravity
- Numerical Studies of Reacting Flow over Flat Walls with Fuel Injection : Part2:Triple-Structure / Stabilization of a Diffusion Flame
- Numerical Studies of Reacting Flows Over Flat Walls with Fuel Injection : Part 1, Velocity Anomaly and Hydrodynamic Instability
- In vitro activity of meropenem and four other antibiotics against 554 clinical strains obtained from Beijing in 1999
- In-vitro antibacterial activities of cefpiramide and other broad-spectrum antibiotics against 440 clinical isolates in China
- MeCP2 knockdown reveals DNA methylation-independent gene repression of target genes in living cells and a bias in the cellular location of target gene products
- The Essential Role of Histone H3 Lys9 Di-Methylation and MeCP2 Binding in MGMT Silencing with Poor DNA Methylation of the Promoter CpG Island
- Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat
- Hydrogen-Induced Degradation in Mn-Co-Ni-O Negative-Temperature-Coefficient Thermistors
- On the Geometric Equivalence of the Discrete Integrable Modified Heisenberg Ferromagnet Model(General)
- Clinical application of an enzyme-linked immunosorbent assay detecting hepatoma-specific γ-glutamyltransferase
- Effects of NaCl and silicon on ion distribution in the roots, shoots and leaves of two alfalfa cultivars with different salt tolerance(Plant Nutrition)
- Effect of Heat Release on the Behavior of Reacting Mixing Layer
- Artifacts Caused by Dental Metallic Materials on MR Images
- G314 AN EXERGY ANALYSIS OF THE LiBr-WATER TRIPLE-EFFECT ABSORPTION-TYPE REFRIGERATOR(Absorption heat pump)
- Early Postoperative Heterotopic Omental Ossification : Report of a Case
- Resistance of staphylococcal enterotoxin B-induced proliferation and apoptosis to the effects of dexamethasone in mouse lymphocyte cultures
- Adipose tissue-targeted 11β-hydroxysteroid dehydrogenase, type 1 inhibitor protects against diet-induced obesity
- Inhibition of Phosphoinositide 3-Kinase Potentiates Relaxation of Porcine Coronary Arteries Induced by Nitroglycerin by Decreasing Phosphodiesterase Type 5 Activity
- Early intensive insulin therapy attenuates the p38 pathway in the renal cortex and indices of nephropathy in diabetic rats
- Hydrogen Peroxide Enhances Vasodilatation by Increasing Dimerization of cGMP-Dependent Protein Kinase Type Iα
- B-cell epitope KT-12 of enterohemorrhagic Escherichia coli O157:H7 : a novel peptide vaccine candidate