Fluorophotometric Determination of Hydrogen Peroxide and Other Reactive Oxygen Species with Fluorescein Hydrazide (FH) and Its Crystal Structure

スポンサーリンク

概要

• 論文の詳細を見る

• Methods for the fluorophotometric determination of hydrogen peroxide (H_2O_2) and other reactive oxygen species (ROS) were proposed by using the fluorescence reaction between H_2O_2 or other ROS and fluorescein hydrazide (FH). In the determination of H_2O_2, the calibration curve exhibited linearity over the H_2O_2 concentration range of 2.1-460ng ml^<-1> at an emission wavelength of 527nm with an excitation of 460nm and with the relative standard deviations (n=6) of 4.06%, 1.78%, and 2.21% for 3.1ng ml^<-1>, 30.8ng ml^<-1>, and for 308ng ml^<-1> of H_2O_2, respectively. The detection limit for H_2O_2 was 0.7ng ml^<-1> due to three blank determinations (ρ=3). The calibration curves for ROS-related compounds were also constructed under the optimum conditions. This method was successfully applied in the assay of H_2O_2 in human urine. In addition, we performed the characterization of FH, and interesting information was obtained with regard to the relationship between the chemical structure and fluorescence.

• 社団法人日本薬学会の論文
• 2008-07-01

著者

• DOI Mitsunobu

  Osaka University of Pharmaceutical Sciences

• NAKAHARA Ryosuke

  Osaka University of Pharmaceutical Sciences

• FUJIMOTO Tsuyoshi

  Osaka University of Pharmaceutical Sciences

• MORITA Kanako

  Osaka University of Pharmaceutical Sciences

• YAMAGUCHI Takako

  Osaka University of Pharmaceutical Sciences

• FUJITA Yoshikazu

  Osaka University of Pharmaceutical Sciences

• Fujita Yoshikazu

  Osaka College Of Pharmacy

• Yamaguchi Takako

  Osaka Univ. Pharmaceutical Sci. Osaka Jpn

• Yamaguchi Takao

  Osaka University of Pharmaceutical Sciences

• Fujita Yoshikazu

  Osaka College of Pharmaceutical Sciences

関連論文

• Computational Study on Secondary Structure of Oligopeptides Containing α,α-Disubstituted α-Amino Acids
• Controlling 3_-Helix and α-Helix of Short Peptides in the Solid State
• Controlling Helical Secondary Structures by Cyclic α,α-Disubstituted Amino Acids Having Side-Chain Chiral Centers
• Computational Study on Helical Structures of Oligopeptides Containing Chiral Cyclic α,α-Disubstituted α-Amino Acids
• Computational Study on Conformation of Oligopeptides Containing Chiral Cyclic α,α-Disubstituted α-Amino Acids
• Chiral Cyclic α,α-Disubstituted α-Amino Acids Bearing Two Chiral Centers and Conformation of Their Peptides
• Computational Study on Conformation of Oligopeptides Controlled by Side Chain Chiralities of α-Amino Acids
• Relationship between α-Amino Acid Chiral Center and Helical Secondary Structure of Its Oligopeptides
• Controlling the Helical Screw Sense of Oligopeptides by Chiral Cyclic α, α-Disubstituted α-Amino Acid
• Four Guaianolides from Sinodielsia yannanensis
• Crystal Structures of Acetyl Gramicidin S Analogues
• Structual Analyses for Asymmetric Derivatives of Ascidiacyclamide by X-ray Diffraction and NMR Spectroscopy
• Crystal Structure of Hybrid Dipeptide, (2-Carboxyethyl)-cytosine-1-yl-L-threonine Monohydrate
• Crystal Structure of Hybrid Dipeptide, Cytosinyl-L-tyrosine
• Conformation and Sodium Coordinate Forms of an Endothelin Inhibitor BQ123・Na, Cyclo(-D-Trp-D-Asp^~-Pro-D-Val-Leu-)・Na^+, in the Crystal
• Crystal Structure of 2-[N-(t-Butoxycarbonyl)amino]-4-(thymin-1-yl)-butyric Acid Methyl Ester
• Polymorphism and C-H…O Interaction of Wortmannin, a Phosphatidylinositol 3-Kinase Inhibitor
• Crystal Structure of 3, 4-Dihydroxy-6-fluoro-DL-phenylalanine Monohydrate Used as a Positron Emission Tomography Imaging Ligand
• Fluorophotometric Determination of Hydrogen Peroxide and Other Reactive Oxygen Species with Fluorescein Hydrazide (FH) and Its Crystal Structure
• Absolute Stereostructures of Cell-adhesion Inhibitors, Peribysins A, E, F and G, Produced by a Sea Hare-derived Periconia sp
• Hydrogen Bond between Water and the Phenyl Ring in the Structure of a Dipeptide H-Phe-Leu-NH_2 at 90 K and the Structure-based Energy Estimations
• Interaction Modes between N7-Quarternized Guanine and Cytosine-Containing Dipeptides
• Crystal Structure of Hybrid Dipeptide, Uracil-1-yl-(2-carboxyethyl)-glycine
• Crystal Structure of Cytosine and Alanine Hybrid Dipeptide, Cytosine-1-yl-(2-carboxyethyl)-L-alanine
• Spectroscopic Investigation on the Interaction of NCA0424,a Potent Antitumor Indoloquinoxaline Derivative, with DNA
• Thermodynamic Effect of Commplementary Hydrogen Bond Base Pairing on Aromatic stacking Interaction in the Guanine-X-Trp Complex (X=Adenine, Guanine, Cytosine, Thymine)
• SPECTROPHOTOMETRIC DETERMINATION OF VANADIUM WITH GALLEIN AND SODIUM LAURYLSULFATE
• Spectrophotometric Determination of Spermine and Related Compounds Using o-Hydroxyhydroquinonephthalein and Manganese(II)
• SPECTROPHOTOMETRIC DETERMINATION OF BERBERINE CHLORIDE IN PHARMACEUTICAL PREPARATIONS
• SPECTROPHOTOMETRIC DETERMINATION OF GERMANIUM(IV) USING ο-HYDROXYHYDROQUINONEPHTHALEIN AND CETYLPYRIDINIUM CHLORIDE
• COLOR REACTION BETWEEN POLYAMINES AND ο-HYDROXYHYDROQUINONEPHTHALEIN-COPPER(II) COMPLEX
• SPECTROPHOTOMETRIC DETERMINATION OF TUBOCURARINE CHLORIDE USING COLOR REACTION WITH o-HYDROXYHYDROQUINONEPHTHALEIN, ZlRCONIUM(IV) AND FLUORIDE IONS
• Spectrophotometric Determination of Biologically Active Thiols with Eosin, Silver(I)and Adenine
• Spectrophotometric Determination of Ascorbic Acid with Iron(III) and p-Carboxyphenylfluorone in a Cationic Surfactant Micellar Medium
• Spectrophotometric Determination of Gold(III)by an Association Complex Formation between Gold-Thiamine and Phloxine
• Spectrophotometric Determination of Ethylenediaminetetraacetic Acid and Its Related Compounds with p-Carboxyphenylfluorone, Titanium(IV) and Hydrogen Peroxide^1
• Improved o-Sulfophenylfluorone-Titanium(IV) Complex Method for Total Urinary Protein Assay
• Determination of Glycyrrhizinic Acid Based on a Color Reaction with o-Sulfophenylfluorone-Iron(III)Complex
• SPECTROPHOTOMETRIC DETERMINATION OF SULPYRINE USING ο-HYDROXYHYDROQUINONEPHTHALEIN AND CERIUM(IV)
• SPECTROPHOTOMETRIC DETERMINATION OF RESERPINE IN PHARMACEUTICAL PREPARATIONS
• SPECTROPHOTOMETRIC DETERMINATION OF PAPAVERINE WITH o-HYDROXYHYDRO-QUINONEPHTHALEIN AND URANIUM(VI)
• COLOR REACTION BETWEEN PYROGALLOL RED-MOLYBDENUM (VI) COMPLEX AND PROTEIN
• SPECTROPHOTOMETRIC DETERMINATION OF CHLORPHENIRAMINE MALEATE BY COMPLEX FORMATION WITH o-HYDROXYHYDROQUINONEPHTHALEIN, TITANlUM(IV) AND FLUORIDE IN THE PRESENCE OF SODIUM DODECYL SULFATE
• SPECTROPHOTOMETRIC DETERMINATION OF ISONIAZID USING FORMATION OF o-HYDROXYHYDROQUINONEPHTHALEIN-PALLADIUM(II)-CETYLTRIMETHYL-AMMONIUM CHLORIDE COMPLEX
• SPECTROPHOTOMETRIC DETERMINATION OF CHLORPROMAZINE HYDROCHLORIDE USING THE COLOR REACTION WITH PALLADIUM(II) AND o-HYDROXYHYDRO-QUINONEPHTHALEIN
• SPECTROPHOTOMETRIC DETERMINATION OF THIOUREA USING o-HYDROXYHYDROQUINONEPHTHALEIN AND PALLADIUM(II)
• SPECTROPHOTOMETRIC DETERMINATION OF MANGANESE(II) USING o-HYDROXY-HYDROQUINONEPHTHALEIN AND ZEPHIRAMINE
• Computational Study on Helical Structure of α,α-Disubstituted Oligopeptides Containing Chiral α-Amino Acids
• CHEMICAL AND BIOLOGICAL PROPERTIES OF HYDROXO-BRIDGED DINUCLEAR COMPLEXES OF ANTICANCER PLATINUM (II) COMPLEXES
• Design and Synthesis of Chiral Cyclic α,α-Disubstituted Amino Acid Having Azido Functions, and Its Oligopeptides
• Spectrophotometric Determination of Uric Acid Based on Fading of o-Hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethyl-ammonium Complex
• Spectrophotometric Determination of Glucosamine and Its Analogous Amino Sugars with o-Hydroxyhydroquinonephthalein and Palladium(II)
• Spectrophotometric Determination of Cobalt(II) and Cyanocobalamin with Vanillilfluorone and Its Applications
• Spectrophotometric Determination of DNA Binding Protein, Histone, with 3, 4, 5, 6-Tetrafluoro-2-carboxyphenylfluorone and Manganese(II)^1
• Spectrophotometric Determination of Quinolone Antibiotics by an Association Complex Formation with Aluminum(III) and Erythrosin
• Spectrophotometric Determination of Anthracycline Anticancer Agents with Aluminum(III) and Chromazurol S in a Nonionic Surfactant Micellar Medium
• Determination of Trace Aluminum by Fluorescence Quenching with m-Carboxyphenylfluorone as Analytical Reagent
• Design of Short α-Helical Peptides for Transcriptional Inhibitor of Nuclear Receptor
• Computational Study on Conformation of Oligopeptides Containing α,α-Disubstituted Amino Acids
• Stabilized α-Helical Peptide-Catalyzed Asymmetric Epoxidation of Chalcone Derivatives
• KINETIC AND EQUILIBRIUM STUDIES ON DI-μ-HYDROXO DIMER FORMATION OF ANTICANCER PLATINUM (II) COMPLEXES BY POTENTIOMETRIC METHOD
• Conformational Studies of Diastereomeric -Leu-Aib- Peptides
• Spectrophotometric Determination of Copper(II) with o-Bromophenylfluorone
• Synthesis, Spectral Study and Crystal Structure of a Fluorescein Derivative, p-Methoxycarbonylphenyl Fluorone
• Spectrophotometric Determination of Aluminum with m-Carboxyphenylfluorone, a Novel Chemical Probe, and Its Application
• Spectrophotometric Determination of Urinary Protein with o-Sulfophenylfluorone-Metal Complex
• Design and Synthesis of Conformational Freedom Restricted Endomorphin-2 Analogues
• Design of Stable Helical Peptides
• Design of Stapled Helical Peptides for Catalytic Enantioselective Epoxidation of α,β-Unsaturated Ketones
• Synthesis and Conformational Analysis of Peptides Composed of Chiral Five-Membered Ring Amino Acids
• Spectrophotometric Determination of Micro Amounts of Albumin Using Zirconium(IV) and Xylenol Orange
• Fluorophotometric Determination of Histone with 3,4,5,6-Tetrafluoro-2-carboxyphenylfluorone–Manganese(II) Complex and Its Characterization
• Selective fluorimetric determination of trace amounts of cobalt(II) with the system of fluorescein-hydrogen peroxide-tiron.
• The application of diarylamine compounds in analytical chemistry. 1. Fluorescence reactions among 4-(4-methyl-2-quinolyl)aminosalicylic acid, cobalt(II) and hydrogen peroxide, and the fluorimetry of cobalt(II) by using its fluorescence reaction.
• The sensitive and selective determination of palladium(II) based on ternary-complex formation with phloxine and thiamine.
• Color reaction between indium(III) and o-hydroxyhydroquinonephthalein, and its application.
• The spectrophotometric determination of trace vanadium with 3,4,5,6-tetrachlorogallein.
• Color reaction among pyrogallol red, thorium(IV) and samarium(III), and its application to the determination of these metals.
• Spectrophotometric determination of gentamicin by using a membrane filter preconcentration technique with o-hydroxyhydroquinonephthalein and uranium (VI).
• Determination of titanium(IV) using o-hydroxyhydroquinonephthalein and Tween 20.
• The color reaction of purine bases by ternary-complex formation with eosin and silver(I).
• Application of Xanthene Derivatives for Analytical Chemistry, Part LXXXVI. Determination of Rhodium by Third-Derivative Spectrophotometry with o-Hydroxyhydroquinonephthalein in the Presence of N-Hexadecylpyridinium Chloride.
• The determination of 5-iodouracil using the o-hydroxyhydroquinonephthalein-palladium(II) complex.
• Synthesis of Chiral Five-membered Ring Amino Acids wtih an Azido Group, and Their Peptides
• Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment(PLANT BIOTECHNOLOGY)

もっと見る 閉じる

スポンサーリンク