Spectrophotometric determination of gentamicin by using a membrane filter preconcentration technique with o-hydroxyhydroquinonephthalein and uranium (VI).

概要

論文の詳細を見る
Color reactions among amino glycoside antibiotics such as gentamicin (GM), a metal ion such as uranium(VI), and o-hydroxyhydroquinonephthalein (Qnph) as a xanthene dye were studied by using a preconcentration technique based on membrane filtration. A simple, new, and highly sensitive spectrophotometric determination of GM has been established by using a preconcentration to collect Qnph-uranium(VI)-GM ternary complex. Beer's law is obeyed over the range 0–5.4 μg of GM in 5 cm3 of dimethyl sulfoxide at 565 nm, and the apparent molar absorptivity for GM is 4.2×105 dm3 cm−1 mol−1 with a Sandell sensitivity of 0.0013 μg cm−2 GM at 565 nm. The procedure is about 2.5-fold as sensitive as the method employing aqueous medium, and is applicable to determination of GM in calf serum.
公益社団法人日本化学会の論文