Spectrophotometric Determination of Cobalt(II) and Cyanocobalamin with Vanillilfluorone and Its Applications
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概要
- 論文の詳細を見る
Spectrophotometric determination of cobalt(II) was accomplished with vanillilfluorone (VF) in the presence of dimethylbenzyltetradecylammonium chloride (Zephiramine, Zep). In the determination of cobalt(II), Beers law was obeyed in the range of 24—470 ng/ml, with an effective molar absorption coefficient (at 575 nm) and relative standard deviation of 1.35×105 l mol−1 cm−1 and 0.66% (n=5), respectively. The composition ratio of the colored complex was determined by the mole ratio and continuous variation methods, and it was found to be Co(II) : VF : Zep=1 : 2 : 4. Analysis of cyanocobalamin by the same procedure showed that cyanocobalamin could be determined in the concentration range of 0.5—0.11 μg/ml using the proposed method.
著者
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YAMAGUCHI Takako
Osaka University of Pharmaceutical Sciences
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FUJITA Yoshikazu
Osaka University of Pharmaceutical Sciences
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Asano Mamiko
Osaka University
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Hoshino M
Nagoya Institute Of Technology Department Of Applied Chemistry
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HOSHINO Mitsuru
Osaka University Pharmaceutical Sciences
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Matsushita Momoka
Osaka University of Pharmaceutical Sciences
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Samma Megumi
Osaka University of Pharmaceutical Sciences
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Fujita Yoshikazu
Osaka College Of Pharmacy
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MIYAMOTO Mika
Osaka University of Pharmaceutical Sciences
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Matsushita Momoka
Osaka Univ. Of Pharmaceutical Sciences
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Yamaguchi Takako
Osaka Univ. Pharmaceutical Sci. Osaka Jpn
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Hoshino Mitsuru
Osaka University of Pharmaceutical Sciences
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Fujita Yoshikazu
Osaka College of Pharmaceutical Sciences
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