初代ラット大脳皮質由来神経細胞の凍結保存条件に及ぼす凍結速度依存性(平成19年度第53回低温生物工学会研究報告)

概要

論文の詳細を見る
Developments of cryopreservation techniques on primary rat cortical cells were carried outto observe the effect of cooling rates. Primary rat cortical cells were dispersed in thecryopreserving medium including 5 vol% dimethylsulfoxide, and then they were frozen byvarious cooling rates ranged from -3 K/min to -150 K/min. The survival ratio was estimatedqualitatively by using the phase-contrast microscope. The feasibility of the neural activity wasverified by measuring its spontaneous action potential with a multi-electrode array system.Based on these characterizations, the lower cooling rate of the dispersed sample was morefeasible for the cryopreservation of the primary rat cortical cells.
低温生物工学会の論文
2007-12-30